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제주단지무와 관동여름무의 생육시기별 유용성분 변화

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Alternative Title
Changes in Functional Metabolites at Different Growth Stages of Danji radish and Gwandongsummer radish
Abstract
Functional metabolites were screened in native species of radish at different
growth stages to broaden consumer market. Both Danji radish and
Gwandongsummer radish were harvested periodically in 80, 100, 120, 150 and 180
days after sowing. Their roots and leaves were investigated for ally
isothiocyanate(AITC), sulforaphane, total polyphenol compounds(TPC), total
flavonoid compounds(TFC), minerals, antioxidative activity, myrosinase activity,
and antibacterial activity.
Danji radish produced 2~5 fold more leaves than Gwandongsummer radish. The
highest amount of leaves 902 g/radish was obtained at 100 days' cultivation. The
contents of AITC showed differences according to harvest time and species.
The maximum levels were 0.94mg/g in roots of Danji radish and 1.02mg/g in
leaves of Danji radish harvested in 100 days after sowing. Meanwhile,
Gwandongsummer radish that were harvested contained only 0.69mg/g in roots and
0.72mg/g in leaves. The contents of sulforaphane were also higher in Danji
radish roots (12.6ppm) and leaves (7.31ppm) than in Gwandongsummer radish roots
(6.46ppm) and leaves (6.34ppm). The enzyme myrosinase which catalyzes AITC
production through hydrolysis of glucosinolate was detected 30~42 unit in
leaves of Danji radish which was higher than 20~23 unit in leaves of
Gwandongsummer radish. Above 300 ppm of both TPC and TFC were contained
in radish leaves, and leaves had more TPC and TFC than roots. The leaves of
Danji at 100 days' cultivation showed the highest contents. Macro-mineral
contents in radish were K>Ca>Mg>P in descending order. Among those,
potassium content with 2~4% was 10 times higher than that of any other
mineral. Iron (Fe) out of four micro-elements(B, Zn, Fe and Cu) showed the
richest content 498.70ppm in leaves of Danji radish, whereas leaves of
Gwandongsummer radish had only 116.90ppm of iron. The antioxidative activity
of radish was investigated indirectly by measuring radical-scavenging activity.
The radical-scavenging activities of Danji radish were 42.32% for
2-diphenyl-1-picrylhydrazyl and 57.47% 2,2'-azino-bis3-ethylbenzenothiazolin-6-sulfonic acid, respectively, compared with Trolox.
Extracts of radish showed considerable antibacterial activity. Among those the
extract from Danji roots had the strongest activity.
This study suggests that Danji radish is more valuable and suitable as leave
sources than Gwandongsummer radish, and it has high potential as functional food
material.
Author(s)
문애경
Issued Date
2015
Awarded Date
2015. 8
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000007222
Alternative Author(s)
Moon, Ae Kyoung
Department
대학원 식품공학과
Table Of Contents
Ⅰ. 서 론 3
Ⅱ. 재료 및 방법 7
1. 생육 특성 조사 7
2. 실험 재료 7
3. 분석용 시료 조제 7
4. 유용성분 분석 8
1) Ally isothiocyanate 분석 8
2) Sulforaphane 분석 10
3) Myrosinase 활성 10
4) 총 폴리페놀함량 분석 12
5) 총 플라보노이드 함량 분석 12
6) 무기성분 분석 13
5. 항산화 및 항균 활성 13
1) 2,2-diphenyl-1-picrylhydrazyl radical 소거능 13
2) 2,2'-azino-bis 3-ethylbenzenothiazolin-6-sulfonic acid radical 소거능 14
3) 항균 활성 14
6. 통계분석 14
Ⅲ. 결과 및 고찰 15
1. 생육 특성 15
2. 유용성분 분석 18
1) Ally isothiocyanate 함량 변화 18
2) Sulforaphane 함량 26
3) Myrosinase 활성 변화 28
4) 총 폴리페놀과 플라보노이드 함량 변화 29
5) 무기성분 함량 변화 31
3. 항산화 및 항균 활성 33
1) 항산화 활성 변화 33
2) 항균 활성 변화 35
요 약 37
Ⅳ. 참고문헌 39
Degree
Master
Publisher
제주대학교 대학원
Citation
문애경. (2015). 제주단지무와 관동여름무의 생육시기별 유용성분 변화
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General Graduate School > Food science and Engineering
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