Agrobacterium tumefaciens를 이용한 단자엽 식물의 형질전환

Abstract

Monocotyledonous plants including many species are food and economically important crops. Recently many kinds of functional genes were transformed in various monocot plants through Agrobacterium tumefacience. In the present study ABF3 and human lactoferrin genes are transformed into rice (Oriza sativa L.) and oat phytochrome A gene is transformed into zoysiagrass (Zoysia japonica steud.) through modified and improved method of Agrobacterium- mediated transformation. ABF3 gene has the function against environmental stress. In this part over 30 transgenic plants are established. Among tested lines, all transgenic plant shows 3:1 segregation ratio in T1 progenies. The transgenic plants showed higher tolerance than wild-type plants against cold stress. Human lactoferrin (HLF) is antimicrobial compound of breast-fed infants. HLF gene is transformed using herbicide tolerance gene (bar) for plant selection marker. In this part over 40 transgenic plants are established and confirmed stable gene integration and herbicide tolerance. Transformed HLF gene is stably inherited to their progeny and that protein is expressed in progeny plants. Zoysiagras (Zoysia japonica steud.) takes much cost for weed control and mowing to manage beautiful grass field. For suppress shade-avoidance of the plants, oat phytochrome A mutant gene is transformed using herbicide tolerance gene (bar) for plant selection marker. Integration of gene and expression of protein in transgenic plants are confirmed through molecular analysis. Oat phytochrome A mutant S598A gene transformed plant were shown higher suppressed elongation than wild-type plants. Through this investigation, several functional genes are transformed at one time successfully using modified Agrobacterium-mediated transformation in two species of monocot plants.