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Induction of apoptosis in SNU-16 human gastric cancer cells by the chloroform fraction of extract of dangyuja(Citrus grandis Osbeck)and guava(Psidium guajava L.) leaves

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Alternative Title
당유자 잎과 구아바 잎 Chloroform 분획물에 의한 human 위암 세포주인 SNU-16 세포에서의 apoptosis 유도
Abstract
The anticancer effect of dangyuja (Citrus grandis Osbeck) and guava (Psidium guajava L.) leaves extract was investigated using SNU-16 human gastric cancer cells. Maximum cytotoxicity was observed using the chloroform fraction (CF) of the extract. Cell death was dose-dependent and was characterized by apoptotic body formation and DNA fragmentation. Flow cytometric analysis showed that treatment of chloroform fraction of dangyujaleaves (CFD) and chloroform fraction of guava leaves (CFG) resulted in a marked accumulation of cells in sub-G1 phase. The induction of apoptosis was confirmed by caspase-3 activity assays and by immunoblotting using antibodies against Bid, Bcl-2, Bax, poly (ADP-ribose) polymerase (PARP), caspase-9, caspase-8, caspase-7, and caspase-3. Together, our results provide the first evidence that the CFD and CFG extracts induces apoptosis in SNU-16 cells. Our findings may lead to new strategies for the treatment of human gastric cancer.
Author(s)
Moon, Jeong Yong
Issued Date
2009
Awarded Date
2009. 2
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000004678
Alternative Author(s)
문정용
Affiliation
제주대학교 대학원
Department
대학원 생명공학과
Advisor
김소미
Table Of Contents
1. INTRODUCTION = 1
2. MATERIALS AND METHODS = 3
2.1. Reagents = 3
2.2. Preparation of the leaf extracts = 3
2.3. Cell culture = 4
2.4. Cell viability = 4
2.5. Microscopic observation of cellular morphology = 5
2.6. Analysis of DNA fragmentation = 5
2.7. Flowcytometric analysis = 5
2.8. Immunoblot analysis = 6
2.9. Caspase-3 activity assay = 7
2.10. GC and GC-MS analysis = 7
2.11. Statistical analtsis = 8
3. Results and Discussion = 9
3.1. Effect of the CF on cell viability = 9
3.2. Inducation of apoptosis by the CFD and CFG = 15
3.3. Effects on cell cycle progression = 18
3.4. Western blot analysis for apoptosis-related proteins = 22
3.5. Composition of chloroform fraction = 27
References = 30
ABSTRACT IN KOREAN = 36
ACKNOWLEGEMENT = 37
Degree
Master
Publisher
제주대학교 대학원
Citation
Moon, Jeong Yong. (2009). Induction of apoptosis in SNU-16 human gastric cancer cells by the chloroform fraction of extract of dangyuja(Citrus grandis Osbeck)and guava(Psidium guajava L.) leaves
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Faculty of Biotechnology > ETC
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