개가시나무, 산딸나무 및 볏붉은잎의 활성성분 규명

Abstract

For the development of functional cosmetic additives, we have screened plant in Jeju island on DPPH inhibition activities. Quercus gilva, Cornus kousa and Callophyllis japonica distributed in Jeju has shown inhibition activities on free radical scavenging test. In this study, five antioxidative components were isolated from Quercus gilva, Cornus kousa and there structures were identified by 1D-NMR (¹H-NMR, ^(13)C-NMR, DEPT) and 2D-NMR (HMQC, HMBC) techniques. Isolated compounds were identified as catechin, epi-catechin, tyrosol, tilliroside, kaempferol, astragalin, quercetin-3-O-β-D-glucopyranoside, bis(3- chloro-2-hydroxypropyl)ether, 2,2-Bis[4-(glycidyloxy)phenyl]propane. their anti -oxidative activities were examined. RC_(50) of DPPH radical scavenging for catechin, epi-catechin, kaempferol, astragalin, quercetin-3-O-β-D-glucopyranoside were measured at the concentration of 7.89㎍/mL, 8.70㎍/mL, 9.27㎍/mL, 48.33㎍/mL, 8.01㎍/mL respectively. These radical scavenging activities were more potent than that of L-ascorbic acid.