개의 피부 감염창 치유에 대한 녹차 추출물의 국소도포 효과

Abstract

The purpose of this study is to assess healing effect of the green tea on Staphylococcus contaminated wound. On the back of 7 dogs, 6 full-thickness skin wounds (2cm × 2 cm) were made and Staphylococcus intermedius was inoculated. Each wound was applied with the 2% (experimental group Ⅰ), 1% (experimental group Ⅱ) extract of green tea and normal saline (control group) after inoculation. The wound contraction rate of the experimental group Ⅰ and Ⅱ was higher than that of the control group in entire period. And from the 6^(th) to 22^(nd) day, the wound contraction rate of the experimental group Ⅰ and Ⅱ was significantly higher than that of the control group(p<0.05). The occupation rate of wound healing in the experimental group Ⅰ was higher than that of the control group from 0 to 9^(th) day except from 1^(st) to 2^(nd) day and also higher than that of the experimental group Ⅱ from 0 to 3^(rd) day except from 1^(st) to 2^(nd) day. And the occupation rate of wound healing in the experimental group Ⅱ was higher than that of the control group from 1^(st) to 8^(th) day except from 2^(nd) to 3^(rd) day. According to concentration of green tea extract increased, the wound contracted more vigorously in earlier period. The tensile strength of the experimental group Ⅰ and Ⅱ was significantly higher than that of the control group(p<0.05). And that of the experimental group Ⅰ was also higher than in the experimental group Ⅱ(p<0.05). The number of S. intermedius in the experimental group Ⅰ and Ⅱ was decreased more rapidly than that of the control group. From the 4^(th) to the 11^(th) day, the number of S. intermedius in the experimental group Ⅰ was significantly lower than that of the experimental group Ⅱ and the control group. And from the 4^(th) to the 7^(th) day, the number of S. intermedius in the experimental group Ⅱ was significantly lower than that of the control group. The histopathological findings of the experimental group Ⅰ and Ⅱ were similar to those of the control group on the 1^(st) and 4^(th) day. On 14^(th) day, reepithelialization was completed in the experimental group Ⅰ and Ⅱ except the control group. Higher wound contraction rate, better bacteriocidal action, higher tensile strength and better histopathological findings were observed in the experimental group Ⅰ and Ⅱ than in the control group. These results suggest that the topical application of green tea extract can promote contaminated wound healing in dogs.