갯기름나물 뿌리에서 항암물질의 분리 및 구조동정

Abstract

Peucedanum japonicum Thunb. (Umbelliferae) is a wild plant of white flower blooming from June and August at the coast in Korea. This plant is well known to have antifungal, stamina, antifood poisoning and antiphlegm, anticancer agent. This study was conducted to isolate anticancer substance from P. japonicum roots and to evaluate its anticancer activity. Roots of P. japonicum were air-dried, chopped and extracted with 100% MeOH. The MeOH extract has been partitioned with CHCl3 and BuOH. CHCl3 layer were purified by repeated column chromatography and recrystallization. As results, two compounds isolated from CHCl3 layer were identified as (10E)1,10-heptadecadiene-4,6-diyne-3,8,9-triol (Comp. I) and anomalin (Comp. II) by NMR spectroscopic analysis. Quantitative analysis using UPLC (Ultra Performance Liquid Chromatography) revealed that roots of P. japonicum contain 0.015 (Comp. I) and 1.69 mg/g (Comp. II). In vitro cytotoxic activity of Comp. I and Comp. II was evaluated against human cancer cell lines; HeLa, HepG2, SNU-16 and AGS by MTT assay. Comp. I showed the most potent cytotoxic activity against HepG2 cell (IC50 = 6.04 ㎍/mL), and Comp. II showed the most potent cytotoxic activity against SNU-16 cell (IC50 = 18.24 ㎍/mL) among the tested human cancer cell lines. However, no significant cell death was observed in CCD-25Lu (Human normal lung fibroblast cell). Thus, these results indicated that the (10E)1,10-heptadecadiene- 4,6-diyne-3,8,9-triol and anomalin may serve as potential phytochemicals with anticancer activity.