난소를 적출한 암컷 쥐에서 CFA로 유발시킨 관절염에 대한 타조한방약제추출액의 효과

Abstract

This study was performed to find out the effect of ostrich extracts with herbs on the arthritis. First, the ostrich extract composition was analyzed. In vitro, I examined the anti-inflammatory effect of ostrich extract combined with herbs in LPS-stimulated murine RAW264.7 cell by proinflammatory cytokines (TNF-α, IL-6) and NO, iNOS concentration. Based on this experiment, I investigated the effect of ostrich extract (OE), herbs extract (HE), ostrich extract with herbs (OEH) on the arthritis induced by CFA in ovariectomised female rats. The analysis of the composition of the ostrich extract and ostrich extract with herbs showed that they contained 56 % unsaturated fatty acid and 44 % saturated fatty acid, the saturated/unsaturated ratio being 0.78 and that no heavy metals such as lead (Pb), arsenic (As), mercury (Hg), cadmium (Cd) were not detected. Ostrich extract with Herbs significantly inhibited cytokines (TNF-α, IL-6) by LPS-stimulation, especially at 800 ㎍/㎖ administration group, compared with ostrich extract and herbs extract. Because of the iNOS inhibition, NO production was significantly low at 200 ㎍/㎖, 400 ㎍/㎖, 800 ㎍/㎖ group of the ostrich extract with herbs, compared with the other two groups. Since ostrich extract with herbs was administrated, the clinical signs of the arthritis rats have begun to improve in two weeks and have significantly improved in four weeks, compared with the other two groups. And the number of WBC has significantly decreased in 6 weeks. Cholesterol concentration was increased at ostrich extract group and ostrich extract with herbs group. HDL concentration was significantly high at OEH group in two weeks, while LDL concentration was significantly high at OEH, OE, HE groups in two weeks compared with the control group. In early arthritis, TNF-α was increased, in OE, HE, OEH groups, TNF-α was significantly low compared with the control group. Estrogen concentration was significantly high at OE, HE, OEH groups in two weeks compared with the control group. The estrogen concentration of OEH group was significantly higher than that of the other two group. In conclusion, ostrich extract with herbs inhibited inflammation-mediated cytokines production and prevented inflammation by arresting NO synthesis. Also, OEH improved the clinical signs of arthritis caused by CFA in ovariectomised famale rat and it was confirmed that OEH was therapeutic effect on the arthritis because of anti-inflammation.