미역쇠(Petalonia binghamiae) 추출물의 인슐린성 작용에 관한 연구

Abstract

In this study, we investigated the anti-diabetic effects of the ethanol extract of Petalonia binghamiae. First of all, the effects of Petalonia binghamiae extracts (PBE) on the adipocyte differentiation were evaluated using 3T3-L1 preadipocytes. Treatment with PBE increased dose-dependently the accumulation of lipid droplet during the differentiation of 3T3-L1 preadipocytes, which were induced by insulin, 3-isobutyl-1-methylxanthine and dexamethasone mixture. Furthermore, the lipid accumulation, the expressions of peroxisome proliferator-activated receptor gamma, adipocyte fatty acid binding protein, insulin receptor substrate 1, and glucose transporter-4 were increased dose-dependently by PBE-treatment in the absence of insulin. Secondly, the anti-hyperglycemic effects of PBE were evaluated using streptozotocin-induced diabetic mice model. PBE (1%) administration significantly decreased blood glucose level, as well as amount of water intake compared with control diabetic mice. Also, glucose tolerance was dose-dependently improved in PBE-treated diabetic mice. PBE administration decreased the serum levels of triglyceride, glutamic pyruvic transaminase and glutamic oxaloacetic transaminase, but increased serum high density lipoprotein cholesterol level compared with control diabetic mice. Taken together, these results suggest that PBE contain phytochemicals which exert anti-hyperglycaemic and insulin-like properties.