산화적인 스트레스로 유도된 세포손상에 대하여 항산화 효소 활성을 통한 Myricetin의 보호 효과

Abstract

We evaluated the cytoprotective effect of myricetin on oxidative stress damaged cells, it was assessed the scavenging effect of reactive oxygen species (ROS) and activities of antioxidant enzymes. Myricetin showed the scavenging effect of 1,1-diphenyl 2-picrylhydrazyl (DPPH ) radicals, and intracellular ROS. In addition, myricetin restored the activity and protein expression of cellular antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) reduced by hydrogen peroxide (H2O2) treatment. H2O2 induced cellular DNA and lipid damages, and myricetin was found to prevent the DNA damage shown by inhibition of DNA tail, and decrease of nuclear phospho-histone H2A.X expression, which are markers for DNA strand breakage and to inhibit membrane lipid peroxidation shown by inhibition of thiobarbituric acid reactive substance (TBARS) formation and of fluorescence intensity of diphenyl-1-pyrenylphosphine (DPPP). These results suggest that myricetin protects cells against H2O2 induced cell damage via inhibition of ROS generation and activation of antioxidant enzymes.

Key words: myricetin; reactive oxygen species; antioxidant enzyme; lipid peroxidation; DNA strand breakage.