제주산 들깨풀(Mosla puntulata Nakai)로부터 분리된 Luteolin의 구조 확인 및 생리활성에 관한 연구

Abstract

To search for the antioxidation activities of 15 plants species obtained in Jeju, 1,1-diphenyl-2-picrilhydrazyl radical scavenging test was performed. Only Mosla punctulata among them showed better radical scavenging activity than vitamin C. The active compound was isolated from the methanol extract of Mosla puntulata, and identified to be luteolin, whose structure was confirmed by 1 dimensional NMR (1H-NMR, 13C-NMR) and 2 dimensional NMR, such as HMQC, HMBC and COSY, data. Luteolin found to be isolated from Mosla puntulata for the first time. Reduction consentration(RC50) of DPPH radical scavenging for luteolin were 3.18 μg/mL. Its radical scavenging activitiy in terms of RC50 was much stronger than vitamin C. The cosmeceutical activities of luteolin were also tested. It slightly inhibited both tyrosinase and elastase activities. The result indicated that luteolin had moderate whitening and anti-wrinkle activities. Since the antioxidation is necessary to prevent skin pigmentation and aging, luteolin purified from Mosla punctulata can be used as functional cosmetics.