제주아그배(Malus micromalus Makino) 추출물의 생리활성에 관한 연구

Abstract

Malus micromalus Makino is a native plant to Jeju Island. In order to evaluate the usefulness of its extract as a functional biomaterial, we investigated several biological activities using the ethanol extract and its fractions from M. micromalus leaves. The ethanol extract showed strong antioxidant activities, such as DPPH scavenging activity (IC50=16.1㎍/㎖), nitric oxide (NO) scavenging activity (IC50=901.2㎍/㎖), superoxide scavenging activity (IC50=42.5㎍/㎖), and xanthine oxidase inhibitory activity (2.7㎍/㎖). Its ethylacetate (EtOAc) fraction showed the strongest antioxidant activities among several fractions. The ethanol extract and its EtOAc fractions dose-dependently inhibited the NO production in a lipopolysaccharide-stimulated murine macrophage RAW 264.7 cells. They inhibited the expression of protein and mRNA of iNOS and COX-2 in a dose- dependent manner. Also, they inhibited tyrosinase activity and melanin content in Melan-a cells, through inhibition of expression of protein and mRNA of tyrosinase and TRP-1. These results suggest that extract of M. micromalus could be used as functional biomaterial in developing a skin whitening agent having the antioxidant and anti-inflammatory activity.