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차풀(Cassia mimosoides var. nomame)로부터 Luteolin의 분리, 동정 및 활성분석

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Alternative Title
Identification and Characterization of Luteolin isolated from Cassia mimosoides var. nomame
Abstract
For the purpose of development of novel cosmeceutical materials from native Jeju plants, this study was carried out to investigate the antioxidative activities and Elastase inhibition by the methanol extract and following fractions of Cassia mimosoides var. nomame. An antioxidative activity of fractions of Cassia mimosoides var. nomame were examined by measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical. An Elastase inhibition test, also was performed to isolate the wrinkle-reducing agents.
Ethyl Acetate fraction of crude methanol extract of Cassia mimosoides var. nomame showed good activity among 4 other organic fractions. The active substance in ethyl acetate fraction was successively isolated with Celite and Sephadex^(TM) LH-20 column chromatography. The purified active substance was identified as Luteolin by ¹H-NMR and ^(13)C-NMR and 2D NMR.
This compound exhibited DPPH radical scavenging activity with the RC_(50) value of 3.86㎍/mL and Elastase inhibition with the IC_(50) value of 53.98㎍/mL.
Considering antioxidative activity on DPPH assay and Elastase inhibition test, Luteolin isolated from Cassia mimosoides var. nomame showed a possibility to be used as a new material for functional cosmeceutical.
Author(s)
김경범
Issued Date
2007
Awarded Date
2007. 2
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000003919
Alternative Author(s)
Kim, Gyoung-Bum
Affiliation
제주대학교 대학원
Department
대학원 화학과
Advisor
이선주
Table Of Contents
Ⅰ. 서론 = 1
Ⅱ. 재료 및 방법 = 7
1. 재료 = 7
2. 시약 및 기기 = 7
3. 실험방법 = 8
3-1. 시료의 추출 및 유효성분 분리 = 8
3-2. DPPH free radical 소거활성에 의한 항산화 활성 측정 = 10
3-3. Elastase 활성저해 측정 = 10
3-4. Tyrosinase 활성저해 측정 = 11
Ⅲ. 실험결과 및 고찰 = 12
1. DPPH free radical 소거활성에 의한 항산화 활성 측정 = 12
2. Elastase 활성저해 측정 = 13
3. Tyrosinase 활성저해 측정 = 14
4. Luteolin의 분리 및 구조 확인 = 15
Ⅳ. 결론 = 24
Ⅴ. 참고문헌 = 25
Degree
Master
Publisher
제주대학교 대학원
Citation
김경범. (2007). 차풀(Cassia mimosoides var. nomame)로부터 Luteolin의 분리, 동정 및 활성분석
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General Graduate School > Chemistry
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