희귀 및 멸종위기 식물 만년콩(Euchresta japonica Benth.)의 종자 발아 및 기내증식에 관한 연구

Abstract

In this study, effects of plant growth regulators on seed germination, callus induction and in vitro proliferation were surveyed, in order to find appropriate culture condition for mass propagation and to discuss habitat conservation or habitat restoration of Euchresta japonica Benth, a rare and endangered wild plant. Germination rate was 86.4% when the seeds were stored in 4℃, whice it was 76.1% when stored in room temperature. Callus induction from stems of seedlings was better on MS medium than on WP medium. Especially, calli were well induced on WP medium containing NAA 1㎎/L and BA 0.5, 1, 2㎎/L, NAA 2㎎/L and BA 0.5, 1㎎/L, NAA 5㎎/L and BA 0.5㎎/L. However, shoots or roots were not differentiated on any media from stems of seedlings. However, when nodes were cultured to produce many shoots, shoots were well formed on NAA 0.1㎎/L and BA 1㎎/L, GA3 0.1㎎/L and BA 1㎎/L, GA3 0.5㎎/L and BA 2㎎/L. Subsequently, root formation from the shoots derived from nodes was stimulated in the medium with 5㎎/L IBA. Specially, roots were well formed when isolated shoots were soaked for 1 min into 500㎎/L IBA solution and cultured in hormone-free WP medium. These results indicate that the treatment with combination of GA3 and BA would be more useful for shoot proliferation of E. japonica Benth. It would be effective, for the root induction from shoots, to soak isolated shoots into a high concentration of IBA and to transfer them on hormone-free WP medium without plant growth regulators. The appropriate conditions which plantlets produced through in vitro propagation could adopt to natural habitats should be established and further research on habitat conservation or restoration is to be performed in near future.