감태로부터 분리된 dieckol이 근육세포 포도당수송 과 간세포 지질축적에 미치는 영향

Abstract

Type 2 diabetes is one of the fastest growing public health problems worldwide resulting from both environmental and genetic factors. It is a metabolic disorder that is characterized by hyperglycemia and a number of complications occurred in different organs and tissues. Ecklonia cava is a kind of brown sea algae and its extract has known to be anti-adipogenic, anti-inflammatory, and so on. However, less has been known about the action mechanism in regulating glucose and lipid metabolism. The present study investigated the effect of polyphenol fraction of Ecklonia cava (ECP) and dieckol (DEK) further isolated from Ecklonia Cava on the glucose and lipid metabolism in skeletal muscle cells and liver cells, respectively. ECP and DEK stimulated glucose uptake as well as the translocation of glucose transporter 4 (Glut4) from cytosol to plasma membrane in L6 muscle cells. ECP and DEK also stimulated PI3 kinase (PI3K)-dependent Akt activity, one of crucial signaling cascades for glucose uptake. Inhibition of PI3K could diminish ECP- and DEK-stimuated glucose uptake and Glut4 translocation. However, ECP and DEK failed to stimulate AMPK. In HepG2 liver cells, ECP and DEK suppressed free fatty acid (FFA)-induced intracellular fat accumulation. AMPK activity was stimulated by DEK. Taken together, ECP and DEK stimulate glucose uptake independent of insulin in skeletal muscle cells through PI3K-Akt pathway, and suppress fat accumulation in liver cells, respectively. From these results, ECP and DEK have a potential to prevent or treat metabolic disorders like as diabetes, nonalcoholic fatty liver diseases and so on