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새우나무 잎 추출물로부터 항산화 및 항균 활성

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Alternative Title
Antioxidantive And Antibacterial Effects of Extract from Ostrya japonica Leaves.
Abstract
Ostrya japonica (Betulaceae) tree distributed in the southern region of Korea. In our continuing efforts to find biologically active compounds from plants in Jeju Island, we became interested in the extract of Ostrya japonica leaves. Repeated column chromatography of chloroform and ethyl acetate soluble fractions in the ethanol extract led to the isolation three compounds, such as 4-hydroxybenzaldehyde (1), quercitrin (2), carpinontriol B (3). All of these compounds were isolated for the first time from Ostrya japonica. In addition, activities of the extract and isolated compounds were monitored.
These structures of isolated compounds were elucidated on the basis spectroscopic data from 1D and/or 2D NMR studies as well as by comparison of the data with the literature values.
The results of the anti-oxidation activity test, indicated that the two compounds, quercitrin (2) and carpinontriol B (3), showed DPPH radical scavenging activity with RC50 of 13.12, 58.16 μg/mL respectively (vitamin C, RC50 : 8.80 μg/mL).
Anti-bacterial effects against six kinds of microorganisms(gram positive-Streptococus pygenes, Streptococcus mutans, Staphylococcus aureus, Bacillus Subtilis, gram negative-Enterobacter aerogenes, Escherichia coli) were measured by paper disc method.
Test results showed that good anti-bacterial activity in ethyl acetate layer and butanol layer.
In conclusion, several phytochemicals Ostrya japonica showed biological activities such as inhibition of anti bacterial and DPPH radical scavenging activities, suggesting that these might provide the leads for anti-bacterial or anti-oxidant cosmeceutical ingredient.
Author(s)
강상훈
Issued Date
2011
Awarded Date
2011. 8
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000005591
Alternative Author(s)
Kang, Sang-Hoon
Affiliation
제주대학교
Department
대학원 화학과
Advisor
이선주
Table Of Contents
List of Schemes ⅲ
List of Tables ⅲ
List of Figures ⅳ
List of Abbreviations ⅴ
Abstract ⅵ

Ⅰ. 서론 1

Ⅱ. 재료 및 방법 4
1. 시약 및 기기 4
2. 재료 4
3. 추출, 분획 및 분리 5
3-1. 새우나무 잎의 추출 및 분획 5
3-2. Compound 1의 분리과정 6
3-3. Compound 2의 분리과정 6
3-4. Compound 3의 분리과정 7
4. 활성 검색 9
4-1. DPPH radical scavenging test 9
4-2. Antibacterial test 11
5. 총 폴리페놀 화합물의 함량 분석 12

Ⅲ. 결과 및 고찰 13
1. 새우나무 잎에서 분리된 화합물의 구조 결정 13
1-1. Compound 1의 구조 해석 13
1-2. Compound 2의 구조 해석 15
1-3. Compound 3의 구조 해석 19
2. DPPH radical scavenging activity 27
2-1. 새우나무 잎 추출물 및 분획물의 활성 27
2-2. 새우나무 잎으로부터 분리된 화합물의 활성 29
3. Antibacterial test 31
3-1. 새우나무 잎 추출물 및 분획물의 활성 31
4. 총 폴리페놀 화합물의 함량 분석 32

Ⅳ. 요약 및 결론 34

Ⅴ. 참고문헌 35
Degree
Master
Publisher
제주대학교 대학원
Citation
강상훈. (2011). 새우나무 잎 추출물로부터 항산화 및 항균 활성
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