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Anti-inflammatory and anti-invasive effects of methanol extract of Hydroclathrus clathratus

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Abstract
.
Author(s)
프라사드
Issued Date
2011
Awarded Date
2012. 2
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000005882
Alternative Author(s)
Prasad Tharanga Jayasooriya
Department
대학원 해양생명과학과
Advisor
김기영
Table Of Contents
List of Figures IV
Chapter I. Methanol Extract of Hydroclathrus clathratus Inhibits Production of Nitric Oxide, Prostaglandin E2, and Tumor Necrosis Factor-α in Lipopolysaccharide - stimulated BV2 Microglial Cells via Inhibition of NF-κB Activity
1. Introduction 1
2. Materials and methods 4
2.1 Preparation of MEHC 4
2.2 Cell culture and MTT assay 4
2.3 Nitric oxide assay 5
2.4 Measurement of PGE2 and TNF- α 5
2.5 Isolation of total RNA and RT-PCR 5
2.6 Western blot analysis 6
2.7 Electrophoretic mobility assay (EMSA) 6
2.8 Statistical analysis 6
3. Results 7
3.1 Effects of MEHC on cell viability 7
3.2 Effects of MEHC on LPS-induced NO and PGE2 production 8
3.3 Effects of MEHC on LPS-induced iNOS and COX-2 mRNA and protein 10
3.4 Effects of MEHC on LPS-induced TNF-α production and mRNA expression 12
3.5 Inhibitory effects of MEHC on LPS-induced NF-κB activity 14
4. Discussion 16
5. Conclusion 19
Chapter II. Methanol Extract of Hydroclathrus clathratus Suppresses MatrixMetalloproteinase- 9 in T24 Bladder Carcinoma Cells by Suppressing the NF-κB and MAPK pathways.
1. Introduction 20
2. Materials and methods 22
2.1 Preparation of MEHC 22
2.2 Reagents and Total polyphenol contents 23
2.3 Cell culture and MTT assay 23
2.4 Isolation of total RNA and RT-PCR 24
2.5 Western blot analysis 24
2.6 Electrophoretic mobility assay (EMSA) 25
2.7 Gelatin substrate gel zymography 25
2.8 Matrigel invasion assay 26
2.9 Statistical analysis 26
3. Results 27
3.1 Effects of MEHC on cell viability 27
3.2 Suppressive effect of MEHC on MMP-9 activity 29
3.3 Inhibitory effect of MEHC on invasion of T24 bladder carcinoma cells 31
3.4 Inhibitory effects of MEHC on TNF-α-induced NF-κB activity 33
3.5 Inhibitory effects of MEHC on TNF-α induced MAPK phosphorylation 36
4. Discussion 37
5. Conclusion 40
References 41
Acknowledgment 50
Degree
Master
Publisher
제주대학교 대학원
Citation
프라사드. (2011). Anti-inflammatory and anti-invasive effects of methanol extract of Hydroclathrus clathratus
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General Graduate School > Marine Life Sciences
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