소사나무 가지로부터 항산화 및 항염 활성 성분 규명

Abstract

In this study, anti-oxidative and anti-inflammatory activities were examined on the extract from Carpinus turczaninowii branches and their biologically active constituents were identified. The repeated column chromatography of ethyl acetate-soluble layer in this ethanol extract led to isolation eight constituents such as β-sitosterol (1), daucosterol (2), betulinic acid (3), pyracrenic acid (4), carpinontriol B (5), carpinontriol A (6), afzelin (7) and quercitrin (8). The structures of these compounds were identified by spectroscopic methods and by comparing their data to those in the literature. As far as we know, all of the compounds except quercitrin (8) were isolated for the first time from this plant. To investigate the anti-oxidative and anti-inflammatory activities of the compounds, DPPH radical scavenging activity, ABTS+ radical scavenging activity and nitric oxide production inhibitory activity in LPS-stimulated RAW 264.7 cell were examined. The total phenolic contents for the extract and solvent layers (n-Hex, EtOAc, n-BuOH, water) were estimated as 20.3, 3.1, 43.7, 35.4 and 13.8 mg (GAE/100 mg) respectively. In addition, the total flavonoid contents were measures as 9.0, 29.4, 14.9 and 2.4 mg (quercetin/100 mg) respectively for the extract and EtOAc, n-BuOH and water fractions. For the anti-oxidation tests, the compounds 4 and 8 showed strong DPPH radical scavenging activities with SC50 of 55.2 and 62.4 μM respectively, whose activities were comparable to a positive control vitamin C (SC50 43.5 μM). In addition, compounds 4, 5, 6 and 8 showed strong ABTS+ radical scavenging activities with SC50 of 34.1, 42.1 45.8 and 29.6 μM respectively, showing comparable activity to vitamin C (SC50 31.6 μM). On the screening of anti-inflammatory activities, the compounds 5 and 6 showed the considerable inhibition on the production of nitric oxide for the RAW 264.7 cell without any cell toxicity. Based on these results, C. turczaninowii branch extract could be potentially applicable as anti-oxidant and anti-inflammatory agent in cosmeceutical and/or pharmaceutical ingredient.