주걱비름 잎절편으로부터의 식물체 재분화 및 대량증식

Abstract

The aim of this study was to establish plant regeneration from leaf explants of globally rare and endangered species Sedum tosaense Makino. The leaf explants of S. tosaense were cultured on the MS medium supplemented with different concentration of BA and NAA for plant regeneration. Callus induction and weight obtained the highest (100%) on MS medium containing 2.0 mg?L-1 BA and 1.0 mg?L-1 NAA. The highest number of shoots, weight of shoots, compactness of shoots were regeneration when callus were cultured on MS medium containing 2.0 mg?L-1 BA and 1.0 mg?L-1 NAA. The axillary bud were cultured on the MS media supplemented with combination of BA and NAA for in vitro mass propagation of S. tosaense . MS medium supplemented with 2.0 mg?L-1 BA and 1.0 mg?L-1 NAA was number of shoots 7.9, weight of shoots 66.9 mg by effective the best for mass propagation. For rooting, MS medium supplemented with and without 2.0 g?L-1 activated charcoal was tested. The optimal results were observed using MS medium supplemented with 2.0 g?L-1 activated charcoal, on which 85.7 (No. of root), length of root 4.6 cm. 1200 ppm CO2 and 350 ppm CO2 were supplide for make certain the effects of CO2 on pre-acclimatization by photoautotrophic culture. Number of leaf, leaf width, leaf thickness, length of shoot, length of root, total weight and chlorophyll contents of 1200 ppm CO2 treatment was estuimented higher than 350 ppm CO2 treatment. The survival rate was 100% in soil acclimatization of regenerated plantlets were mixed culture soil with peat moss and perlite that was the highest culture condition and the greated growth.