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해수 스피룰리나(Spirulina maxima)의 저가 배양을 위한 기초연구

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Abstract
Spirulina sp. which is one of commercially important interest alga with Chlorella sp. and Dunaliella sp. has been produced in the form of pills and spray-dried powder approximately 3000 tons per year in the world. Though Spirulina sp. cultivated in several sites so far, many places have ceased its production owing to high cost of production, so Spirulina sp. is, today, produced in only few countries. The reason for the high cost of production is because of the Zarrouk's medium generally used for cultivating Spirulina sp. which contains a tremendous amount of carbon, nitrogen and phosphorus compounds resulting in the high cost of production, compared to other mediums such as Allen's medium, Bold's Basal Medium, Blue Green Medium-11, F/2 medium for cultivating other microalgae such as Chlorella sp., Haematococcus sp., Scenedesmus sp.
This study was conducted with applying shells and soil to improve efficiency and economic feasibility of spirulina production. Both materials went through chemical treatments in their separate ways, which were the alkali treatment with 0.1 M sodium hydroxide for shells and the acid treatment with 10% hydrogen peroxide for soil. The by-products from these treatment on materials; shells and soil extract, were used in this study. Finally, the shells extract was used as a solvent for the medium and the soil extract was used as metal solution to substitute for that of Zarrouk's medium.
As a result, not only was there much differences in the biomass, yield of chlorophyll-a, C-PC and biochemical compositions between the control group and the experiment group but also it showed 15% cost reduction from Zarrouk's medium. Additionally, two things which that the amount of the carbon content had an effect on the growth of spirulina sp. rather than production of chlorophyll-a and C-phycocyanin and that Spirulina sp. was able to produce chlorophyll-a and C-phycocyanin even without molybdenum (Mo) and copper (Cu) were also shown in this study.
Author(s)
정주영
Issued Date
2013
Awarded Date
2013. 2
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000006225
Alternative Author(s)
Jeong,Joo Yeong
Affiliation
제주대학교 대학원
Department
대학원 해양생명과학과
Advisor
최광식
Table Of Contents
1. 서 론 7
2. 재료 및 방법 14
2.1. 균주 및 배양재료 14
2.2. 배양 환경 14
2.3. 패각의 전처리 14
2.4. 토양의 전처리 15
2.5. 실험 1 (CaCl2 변화량 실험) 15
2.6. 실험 2 (NaHCO3 변화량 실험) 15
2.7. 실험 3 (토양추출물 실험) 16
2.8. 최종실험 (패각과 토양 추출물의 복합실험) 16
2.9. Biomass 17
2.10. 클로로필-a (Chl-a) 함유량 측정 17
2.11. C-Phycocyanin (C-PC) 함유량 측정 17
2.12. 일반성분(Biochemical compositions) 분석 18
3. 결 과 26
3.1. 패각의 전처리 및 검출된 금속이온의 농도 26
3.2. 실험 1 (CaCl2 변화량 실험) 28
3.3. 실험 2 (NaHCO3 변화량 실험) 31
3.4. 실험 3 (토양추출물 실험) 34
3.5. 최종실험 (패각과 토양 추출물의 복합실험) 38
4. 고 찰 42
4.1. 패각의 전처리 및 검출된 금속이온의 농도 42
4.2. 실험 1 (CaCl2 변화량 실험) 44
4.3. 실험 2 (NaHCO3 변화량 실험) 45
4.4. 실험 3 (토양추출물 실험) 45
4.5. 기 타 46
5. 결 론 47
6. 참고문헌 48
Degree
Master
Publisher
제주대학교
Citation
정주영. (2013). 해수 스피룰리나(Spirulina maxima)의 저가 배양을 위한 기초연구
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General Graduate School > Marine Life Sciences
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