해수 스피룰리나(Spirulina maxima)의 저가 배양을 위한 기초연구

Abstract

Spirulina sp. which is one of commercially important interest alga with Chlorella sp. and Dunaliella sp. has been produced in the form of pills and spray-dried powder approximately 3000 tons per year in the world. Though Spirulina sp. cultivated in several sites so far, many places have ceased its production owing to high cost of production, so Spirulina sp. is, today, produced in only few countries. The reason for the high cost of production is because of the Zarrouk's medium generally used for cultivating Spirulina sp. which contains a tremendous amount of carbon, nitrogen and phosphorus compounds resulting in the high cost of production, compared to other mediums such as Allen's medium, Bold's Basal Medium, Blue Green Medium-11, F/2 medium for cultivating other microalgae such as Chlorella sp., Haematococcus sp., Scenedesmus sp. This study was conducted with applying shells and soil to improve efficiency and economic feasibility of spirulina production. Both materials went through chemical treatments in their separate ways, which were the alkali treatment with 0.1 M sodium hydroxide for shells and the acid treatment with 10% hydrogen peroxide for soil. The by-products from these treatment on materials; shells and soil extract, were used in this study. Finally, the shells extract was used as a solvent for the medium and the soil extract was used as metal solution to substitute for that of Zarrouk's medium. As a result, not only was there much differences in the biomass, yield of chlorophyll-a, C-PC and biochemical compositions between the control group and the experiment group but also it showed 15% cost reduction from Zarrouk's medium. Additionally, two things which that the amount of the carbon content had an effect on the growth of spirulina sp. rather than production of chlorophyll-a and C-phycocyanin and that Spirulina sp. was able to produce chlorophyll-a and C-phycocyanin even without molybdenum (Mo) and copper (Cu) were also shown in this study.