사과'히로사키'캘러스 추출물의 기능성 화장품소재로서의 특성

Abstract

In order to investigate functional characterization of callus extracts of apple 'Hirosaki' for cosmetic materials, conditions for callus induction and proliferation were optimized and activities of its extracts regarding to anti-oxidation, wrinkle improvement, hair growth, skin-whitening, and anti-inflammatory effect were tested. For callus induction, segments cut from newly developed stem were used as explants, and cultured on MS solid media containing different concentration of 2,4-D and BA. As the result, yellow and friable callus was induced in combinations of 0.1-1.0 mg/L 2,4-D and 0.1-1.0 mg/L BA. Among those 0.5 mg/L 2,4-D and 1.0 mg/L BA combination was best for callus proliferation as well as callus induction. In the activity test of callus extract to skin improvement, anti-oxidative activity measured by DPPH radical scavenging was very low comparing to positive control of Trolox treatment, and skin-whitening activities which were estimated by inhibition of melanin production in mouse B16 melanoma cell and HeMn cell were not significantly changed. However, the callus extract affected to the activities for wrinkle improvement, hair growth and anti-inflammatory effect. The callus extract showed similar activity with TGF-β used as positive control at 50 ug/mL concentration in the test of wrinkle improvement effect, and increased 40% of hair growth activity measured by growth stimulation of hair follicle dermal papilla cell. Especially, in case of anti-inflammatory effect, callus extract inhibited about 50% of COX-2 expression which was known as response for intermediating inflammation, and about 70% of eotaxin-1 production which was increased by skin inflammation, although NO production was not inhibited. Through this study, it was suggested that the callus induced from stem of apple 'Hirosaki' had active compounds for wrinkle improvement, hair growth and anti-inflammatory effect, and might possess a potential for developing cosmetic materials.