제주도의 콩과식물과 공생하는 질소고정 근류균의 분포에 대한 연구

Abstract

In This study, the diversity and host specificity of the rhizobia having symbiotic relationship will legumes were investigated on the Jeju island. From 33 legume species, a total of 111 strains of the symbiotic rhizobia was isolated. The isolated rhizobia were Restriction Fragment Length Polymorphism (RFLP) with restriction enzymes of HaeⅢ and RsaⅠ band pattern and 16S rRNA gene sequencing. All of 111 isolated strains were divided into 46 different types of RFLP band pattern on the gel electrophoresis, and were identified 19 species, and fifteen strains isolated from 7 species of tree legumes were identified as 7 species of rhizobia and were included in 3 genera; Rhizobium, Bradyrhizobium, and Mesorhizobium. In 26 species of herb legumes, 96 isolated strains were identified as 17 species of rhizobia and were included in 4 genera; Rhizobium, Bradyrhizobium, Mesorhizobium, and Ensifer. Among all isolates, R. leguminosarum was as much as 48 strains. Among 19 species of isolated rhizobia, tan species of B. cytisi, B. iriomotense, B. japonicum, B. jicamae, B. liaoningense, B. pachyrhizi, M. opportunistum, R. lupini, and R. vallis were symbiotic with 2∼5 host plants. And surprisingly, R. leguminosarum showed the relationship with 15 different species of host plants. And nine species were symbiotic with only one host plant. Twelve species were classified as host plants which symbiotic with several rhizobia. Aeschynomene indica showed symbiotic relationship with R. leguminosarum and B. iriomotense, Amphicarpaea edgeworthii with R. leguminosarum and R. multihospitium, Indigofera pseudotinctoria with R. leguminosarum and R. vignae, and Lespedeza thunbergii with R. leguminosarum and R. larrymoorei. Trifolium pratense and Vicia angustifolia were common host of R. leguminosarum and R. vallis, Albizzia julibrissin and Pueraria thunbergiana were the host of B. pachyrhizi and B. jicamae, and B. pachyrhizi and R. lupini respectively. Sophora flavescens was the host of M. huakuii and M. opportunistum, and Cassia mimosoides was that of B. cytisi, B. jicamae, and R. leguminosarum, and Lespedeza cuneata was that of B. cytisi, B. japonicum, R. leguminosarum, R. lupini, and R. mesosinicum, and Kummerowia striata was the partner of many species of rhizobia, B. cytisi, B. japonicum, B. jicamae, B. liaoningense, R. lupini, and R. vitis. The other 21 species of legumes were symbiotic with only one species of rhizobia. The result of this investigation could be the having data for the study of the symbiotic rhizobia with legumes of Jeju Island. Considering that there are over 76 legume species on the Jeju Island, it would be expected that more meaningful study on the symbiotic rhizobia in many aspects could the established if further broad investigation would be done in the future on the Jeju Island.