3T3-L1 세포에서 제주조릿대 (Sasa quelpaertensis Nakai) 추출물과 p-coumaric acid의 지질대사 개선 작용

Abstract

This study was performed to investigate the effects of Sasa quelpaertensis extract and p-coumaric acid on lipid metabolism in 3T3-L1 cells. First of all, the effects of hot water extract from Sasa quelpaertensis leaf (JBE) on the adipogenesis of 3T3-L1 preadipocytes were investigated. JBE suppressed dose-dependently triglyceride accumulation in 3T3-L1 preadipocytes. At molecular level, it reduced the expression of adipogenic transcriptional factors, CCAAT/ enhancer binding protein α (C/EBPα), peroxisome proliferator-activated receptor γ (PPARγ), and fatty acid binding protein (aP2). JBE suppressed the expression of sterol regulatory element binding protein-1c (SREBP-1c) which is expressed during early differentiation stage, as well as its down stream target genes fatty acid synthase (FAS) and adiponectin in a dose-dependent manner. Also, JBE increased phosphorylation of AMP-activated protein kinase (AMPK) which regulates the expression of SREBP-1c, and acetly-CoA carboxylase (ACC) in 3T3-L1 preadipocytes. Next, the effects of p-coumaric acid (CA) which is a major compound contained in JBE, on lipid metabolism in 3T3-L1 cells were investigated. CA was able to suppress triglyceride accumulation only from intermediate stage (4 days after differentiation induction) by reducing expression of C/EBPα and SREBP-1c. In matures 3T3-L1 adipocytes, CA activated AMPK and its substrate, ACC, and increased the gene expression of carnitine plamitoyl transferase-1 (CPT-1). However, CA did not affected glucose uptake and lipolysis in mature adipocytes. Taken together, these results suggest that JBE and CA inhibit adipogenesis of 3T3-L1 preadipocytes and promote fatty acid β-oxidation via AMPK signaling pathway.