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HepG2세포에서 제주조릿대 (Sasa quelpaertensis Nakai)의 지질대사 개선 작용

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Abstract
Jeju dwarf bamboo (Sasa quelpaertensis Nakai) is a native Korean plant that grows only on Mt. Halla on JeJu island. It leaf has been used as traditional medicine due to several beneficial properties. This study was performed to investigate the effects of Jeju dwarf bamboo extract (JBE) and
its constituent, p-coumaric acid (CA) on lipid metabolism in HepG2 cells. The lipid accumulation in HepG2 cells was induced by oleic acid treatment (600 ?M). In lipid accumulated HepG2 cells, JBE and CA increased the phosphorylation of AMP-activated protein kinase (AMPK) and its substrate acetyl-CoA carboxylase (ACC). Real-time PCR analysis showed that JBE and CA increased the expression of carnitine palmitoyl transferase 1A (CPT1A) mRNA, suggesting that they promote the fatty acid β-oxidation. Also, JBE and CA decreased the lipid accumulation through decreasing the expression of the sterol regulatory element-binding protein-1c (SREBP-1c) and fatty acid synthase (FAS). Furthermore, these effects of JBE and CA was reduced by pretreatment of AMPK activation inhibitor, Compound C. Taken together, these results suggest that JBE and CA modulate the lipid metabolism in HepG2 cells via AMPK activation pathway.

Key word : Sasa quelpaertensis Nakai, p-coumaric acid, HepG2 cell, AMP-activated protein kinase (AMPK), fatty acid oxidation, fatty acid synthesis
Author(s)
김정환
Issued Date
2013
Awarded Date
2013. 2
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000006241
Affiliation
제주대학교 대학원
Department
대학원 생물학과
Advisor
김세재
Table Of Contents
Ⅰ.서론 1
Ⅱ.재료 및 방법 4
1.재료 및 추출 4
2.세포 배양 4
3.MTT 분석 4
4.LDH 분석 5
5.지방 축적 유도 6
6.Oil Red O 염색 6
7.Western blot 6
8.RNA 분리 및 Real-time PCR 7
9.TG 함량분석 8
10.통계처리 9
Ⅲ.결과 11
1.HepG2 세포에서 지방축적 유도 11
2.제주조릿대 추출물(JBE)과 p-coumaric acid (CA)의 AMPK 활성화 작용 14
1)지방산 산화 경로 촉진 작용 17
2)지방산 합성 저해 작용 24
3)AMPK 활성화 억제제 실험 29
Ⅳ.고 찰 32
Ⅴ.참 고 문 헌 34
Ⅵ.요 약 40
Degree
Master
Publisher
제주대학교
Citation
김정환. (2013). HepG2세포에서 제주조릿대 (Sasa quelpaertensis Nakai)의 지질대사 개선 작용
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General Graduate School > Biology
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