뽕잎피나무(Tilia taquetii) 잎으로부터 항염 및 주름개선 활성 성분 연구

Abstract

Phytochemical investigation of the ethanol extract from Tilia taquetii leaves resulted in the isolation of nine constituents; phytol (1), chlorophyll c1 (2), epi-catechin (3), isoquercitrin (4), 3,12,13 - trihydroxyoleanan-28-oic acid (5), oleanolic acid (6), arjunolic acid (7), maslinic acid (8), daucosterol (9). The structures of these compounds were confirmed by comparing their spectroscopic data to those in the literature. As far as we know, all of the compounds except epi-catechin (3) were isolated for the first time from this plant. In order to study the skin-related properties for the isolated compounds, bioactivity screenings on anti-oxidation, anti-inflammatory activities and anti-elastase activities were conducted. For the anti-oxidation tests, the compounds 3, 4 and 8 showed strong DPPH radical scavenging activities with SC50 of 5.41, 12.41, 9.75 μg/mL respectively, whose activities were comparable to a positive control vitamin C (SC50 5.71 μg/mL). On the elastase inhibition studies, the compounds 6 and 8 showed stronger activities with IC50 43.6 μg/mL and 89.5 μg/mL whose activities were comparable to a positive control oleanolic acid (IC50 44.94 μg/mL) and ursolic acid (IC50 32.74 μg/mL). Effect on human normal fibroblast function, the ethyl acetate fraction and compounds 7, 8 inhibited collagenase (MMP-1) production in a dose-dependent manner by human normal fibroblast cell. On the screening of anti-inflammatory activities, the compounds 1, 7 and 8 showed the considerable inhibition on the production of nitric oxide for the RAW 264.7 cell without any cytotoxicity. In addition, they reduced LPS-induced secretion of interleukin-6 (IL-6) and interleukin-1β (IL-1β) in RAW 264.7 cell. Also, the ethyl acetate fraction and compound 1, 7 and 8 suppressed the production of NO and IL-6, IL-1β through the decreased expression of iNOS and COX-2, an indication of anti-inflammatory effects. Based on these results, T. taquetii leaves extract could be potentially applicable as anti-aging and anti-inflammatory agent in cosmeceutical and/or pharmaceutical ingredient.