5-Fluorouracil 내성 대장암 세포주의 특성 및 내성극복기전
- Issued Date
- 2013
- Awarded Date
- 2013. 2
- Type
- Dissertation
- Affiliation
- 제주대학교 대학원
- Department
- 대학원 의학과
- Advisor
- 강희경
- Table Of Contents
- 1. ABSTRACT 1
2. INTRODUCTION 3
3. MATERIALS AND METHODS 8
3.1. Reagents 8
3.2. Cell culture 9
3.3. Cell viability assay(Trypan blue staining) 9
3.4. Morphological analysis of apoptosis by Hoechst 33342 staining 10
3.5. Western blot analysis 10
3.6. Co-immunoprecipitation assay 12
3.7. Confocal microscopy 13
3.8. Statistical analysis 13
4. RESULTS 16
4.1. SNU-C5/5-FU stably acquired resistance on 5-fluorouracil 16
4.2. SNU-C5/5-FU has the characteristic of overexpression of phospho-AKT 18
4.3. SNU-C5/5-FU down-regulates E-cadherin and up-regulates phospho-GSK-3B 21
4.4. Phosphorylated AKT led to down-regulation of E-cadherin and
phosphorylation of GSK-3B in SNU-C5/5-FU 24
4.5. SNU-C5/5-FU has characteristic to translocation of B-catenin in cytoplasm and nucleus 27
4.6. Phospho-AKT led to the nuclear translocation of B-catenin 29
4.7. SNU-C5/5-FU has characteristic to overexpression of cyclooxygenase-2 34
4.8. Over-activated NF-kB signaling led to overexpression of COX-2 36
4.9. Phospho-AKT led to activation of NF-kB signaling in SNU-C5/5-FU 39
4.10. Phospho-AKT led to stabilization of survivin via overexpression of COX-2 41
4.11. Phospho-AKT led to expression of survivin in cytoplasm via up-regulation of COX-2 45
4.12. Phospho-AKT led to resistance on apoptosis via stabilization of survivin 47
4.13. Phospho-AKT contributes resistance on 5-fluorouracil in SNU-C5/5-FU 52
4.14. Loss of PTEN was cause of the over-phosphorylated AKT in SNU-C5/5-FU 55
5. DISCUSSION 58
6 REFERENCES 63
- Degree
- Master
- Publisher
- 제주대학교
- Citation
- 김은지. (2013). 5-Fluorouracil 내성 대장암 세포주의 특성 및 내성극복기전
- Appears in Collections:
- General Graduate School > Medicine
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