삼백초 및 돌외로부터 활성성분의 분리 및 구조 규명

Abstract

Saururus chinesis (Lour.) Baill. has been used in Korea as a folk medicine for the treatment of diuretic action. Gynostemma pentaphyllum (THUNB.) MAKINO is also one of the commonly used traditional medical herb for the prevention of various diseases. In our continuing efforts to find biologically active compounds from plants in Jeju Island, we became interested in the extracts of S. chinesis and G. pentaphyllum. Repeated column chromatography of ethyl acetate and butanol-soluble fractions in the extract led to the isolation six compounds such as quercitrin (1), quercetin (2), catechin (3), (6S,9S)-roseoside (4), quercetin 3-O-glucuronide 6"-O-methly ester (5), ombuine 3-O-Rutinoside (6). Compounds 4 and 6 were isolated for the first time from S. chinesis and G. pentaphyllum respectively. The total phenolic contents for the extract and solvent layers (Hex, CHCl3, EtOAc, BuOH, water) were estimated as 30.3, 8.2, 11.4, 49.2, 32.9 and 7.7 mg (GAE/100 mg) respectively. In addition, the total flavonoid contents were measures as 17.2, 7.7, 26.6 and 18.2 (quercetin/100 mg) respectively for the extract, CHCl3, EtOAc and BuOH fractions. For the anti-oxidation activity test, compounds 1, 2, 3 and 5 showed DPPH radical scavenging activity with SC50 of 37.5, 15.8, 9.6, 25.2 μg/mL respectively, whose activities were comparable to a positive control vitamin C (SC50 : 6.9 μg/mL). In addition, compounds 1, 2, 3, 5 and 6 showed ABTS+ radical scavenging activities with SC50 of 64.6, 12.5, 23.4, 19.2 and 78.4 μg/mL respectively, showing comparable activity to vitamin C (SC50 : 11.9 μg/mL). Based on these results, S. chinesis and G. pentaphyllum extracts could be potentially applicable as anti-oxiant cosmeceutical ingredient.