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Isolation of secondary metabolites derived from marine bacteria in Jeju island, Korea and their biological activities

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Abstract
In order to explore marine microorganisms with medical potential, we tried to isolate and identify the marine-derived bacteria from four types of marine samples including float, marine algae, animal, and sponge collected from Jeju Island, Korea. Finally, 21 different strains have been successfully isolated and identified from these samples by 16S rRNA analysis. Then, all strains were cultured in the Marine Broth and were extracted with ethyl acetate, were assessed in a series of bioassays. The results of these assays showed that four extracts inhibited DPPH by more than 50%, four extracts inhibited hydrogen peroxide by greater than 50%, one extract inhibited the growth of HL-60 cells by more than 95%, 14 extracts inhibited nitric oxide production more than 50% and 12 of them without cytotoxicity in LPS-induced RAW 264.7 cells. These data revealed the tested samples to have many and varied activities, Moreover, the large number of samples demonstrating activity in only one or sometimes two assays accentuates the potential of the bacterial strains isolated from marine-derived samples could be the good sources for biological natural products.
Author(s)
Chao Zhang
Issued Date
2013
Awarded Date
2013. 8
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000006464
Affiliation
제주대학교 대학원
Department
대학원 해양생명과학과
Advisor
You-Jin Jeon
Table Of Contents
Introduction 1
Abstract· 14
Materials and methods 15
Results 22
Discussion 34
Conclusion 39
Abstract 41
Materials and methods 43
Results 53
Discussion and conclusion 84
Abstract 88
Materials and methods 89
Results 98
Discussion and conclusion 117
References 120
Acknowledgements 140
Degree
Master
Publisher
제주대학교 대학원
Citation
Chao Zhang. (2013). Isolation of secondary metabolites derived from marine bacteria in Jeju island, Korea and their biological activities
Appears in Collections:
General Graduate School > Marine Life Sciences
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