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Studies on dietary protein hydrolysates for red seabream (Pagrus major) and olive flounder (Paralichthys olivaceus)

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Abstract
이 실험은 참돔(실험 1)과 넙치(실험 2)를 대상으로 사료 내 가수분해 단백질(protein hydrolysates)의 첨가효과를 알아보기 위해 수행되었다. 각 실험에 사용된 실험사료는 어분을 이용한 기초사료(Con)와 기초사료의 어분을 krill hydrolysate concentrate (KH), shrimp hydrolysate powder (SH), tilapia hydrolysate powder (TH)로 각각 4-5% 대체한 3개의 사료로 총 4개의 사료가 제작되었다. 실험 1은 초기평균무게 29.0 ±0.1 g의 참돔을 180 L 원형 플라스틱 수조에 각 실험구당 33마리씩 3반복으로 배치하였다. 실험사료는 1일 2회 만복급이 하였으며 실험은 총 12주간 진행되었다. 실험 2는 초기평균무게 45.5 ±0.2 g의 넙치를 180 L 원형 플라스틱 수조에 각 실험구당 25마리씩 3반복으로 배치하였다. 실험사료는 1일 2회 만복급이 하였으며 실험은 총 5주간 진행되었다. 실험 1의 분석결과 KH와 SH 실험구의 성장 및 사료효율이 대조구에 비해 유의적으로 증가하였다. 실험사료의 단백질 소화율은 가수분해 단백질로 어분을 대체한 실험구(KH, SH, TH)가 대조구에 비해 유의적으로 높았다. Antiprotease 와 superoxide dismutase 활성은 KH 실험구가 대조구에 비해 유의적으로 높았다. Immunoglobulin level은 KH, SH, TH 실험구가 대조구에 비해 유의적으로 높았다. 병원성 균주(Edwardsiella tarda)를 이용한 공격실험 결과 KH와 TH 실험구의 생존율이 대조구에 비해 유의적으로 높았다. 실험 2의 분석결과 SH 실험구의 성장 및 사료효율이 대조구에 비해 유의적으로 증가하였다. 전어체의 단백질 함량은 KH, SH, TH 실험구가 대조구에 비해 높았으며 지방 함량은 TH 실험구가 대조구에 비해 유의적으로 낮았다. Myeloperoxidase와 superoxide dismutase 활성은 SH 실험구가 대조구에 비해 유의적으로 높았으며 immunoglobulin level은 KH 실험구가 대조구에 비해 유의적으로 높았다. 병원성 균주(Streptococcus iniae)를 이용한 공격실험 결과 KH와 TH 실험구의 생존율이 대조구에 비해 유의적으로 높았다. 이상의 결과를 종합해 볼 때 사료 내 가수분해 단백질의 첨가는 넙치와 참돔의 성장과 사료효율을 증진시키고 면역력과 질병저항성을 향상시킬 수 있을 것으로 사료된다.
Two feeding trials were carried out to examine the effects of dietary supplementation of protein hydrolysates on red seabream and olive founder. Two set of experimental diets were prepared in a similar way, a basal fish meal based diet was used as a control and three other diets were prepared by partial replacement of fish meal with krill hydrolysate concentrate, shrimp hydrolysate powder or tilapia hydrolysate powder (designated as Con, KH, SH and TH, respectively). In experiment I, triplicate groups of red seabream (29.0 ± 0.1 g) were fed one of the diets to apparent satiation twice a day for 12 weeks. Growth performance and feed utilization were significantly higher in fish fed the KH and SH diets. Diets with the tested hydrolysates showed significantly higher digestibility of dietary protein compared to the Con diet. Antiprotease and superoxide dismutase activities were significantly increased in fish fed the KH diet compared to those of fish fed the Con diet. Immunoglobulin level was significantly elevated in fish fed diets containing the hydrolysates. Dietary inclusion of KH and TH significantly increased disease resistance of fish against Edwardsiella tarda. In experiment II, Triplicate groups of fish (45.5 ± 0.2 g) were fed one of the diets to apparent satiation twice daily for 5 weeks. Growth performance and feed utilization were significant higher in fish fed SH diet compared to those the other experimental groups. Significantly higher whole-body protein contents were found in all hydrolysate treated groups. Also, significantly increased whole-body dry matter showed in fish fed the SH whereas whole-body lipid significantly decreased fish offered the TH diet. Myeloperoxidase and superoxide dismutase activities were significantly increased in fish fed the SH diet and immunoglobulin did so in fish provided KH diet compare to those of fish fed the Con diet. The results of challenge test revealed the significant increase of fish disease resistance against S. iniae in fish groups fed the KH and TH diets.
The results of two experiments indicate that dietary supplementation of the protein hydrolysates, even to a high fish meal diet, is an efficient strategy to improve growth performance, feed utilization, innate immunity and disease resistance of red seabream and olive flounder.
Author(s)
Bui, Thi Dieu Hien
Issued Date
2014
Awarded Date
2014. 2
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000006673
Affiliation
제주대학교 대학원
Department
대학원 해양생명과학과
Advisor
이경준
Table Of Contents
1. INTRODUCTION 1
EXPERIMENT I. Study on dietary protein hydrolysates for red seabream (Pagrus major) 3
2.1. MATERIALS AND METHODS 4
2.1.1. Experimental diets 4
2.1.2. Fish and feeding trial 8
2.1.3. Sample collection 8
2.1.4. Analytical methods 9
2.1.5. Digestibility test 12
2.1.6. Bacterial challenge 13
2.1.7. Statistical analysis 14
3.1. RESULTS 15
3.1.1. Growth performance 15
3.1.2. Whole body composition 17
3.1.3. Hematological parameters 18
3.1.4. Non-specific immunity parameters 19
3.1.5. Apparent digestibility coefficient 21
3.1.6. Challenge test 22
4.1. DISCUSSION 23
EXPERIMENT II. Study on dietary protein hydrolysates for olive flounder (Paralichthys olivaceus) 28
2.2. MATERIALS AND METHODS 29
2.2.1. Experimental diets 29
2.2.2. Fish and feeding trial 32
2.2.3. Sample collection 32
2.2.4. Bacterial challenge 33
3.2. RESULTS 34
3.2.1. Growth performance 34
3.2.2. Whole body composition 36
3.2.3. Non-specific immunity parameters 37
3.2.4. Challenge test 39
4.2. DISCUSSION 40
5. REFERENCES 43
6. SUMMARY 50
ACKNOWLEDGEMENT 51
Degree
Master
Publisher
제주대학교 대학원
Citation
Bui, Thi Dieu Hien. (2014). Studies on dietary protein hydrolysates for red seabream (Pagrus major) and olive flounder (Paralichthys olivaceus)
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General Graduate School > Marine Life Sciences
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