감귤와인 슬러지를 이용한 미생물 셀룰로오스 생산 및 특성 연구

Abstract

Bacterial cellulose was produced by Acetobacter xylinus subsp. sucrofermentans (ATCC 700178) strains using the mandarin wine sludge broth. It is known that carbon source and nitrogen source plays an important role to produce cellulose. This study was to evaluate that Mandarin wine sludge broth was worthy of a substitute for conventional medium for the producing of BC. The mandarin wine sludge was an inexpensive carbon and nitrogen source with ethanol. It has been actually discarded once the alcohol fermentation was finished in the winery. When the contents of carbon and nitrogen in the sludge were determined, The sludge had less carbon with sugars than YG medium while it had 3 times more nitrogen. BC by Acetobacter in the sludge medium was produced three times more than in YG medium. Bacterial Cellulose(BC) was identified by its morphology and XRD pattern. FESEM data showed similar three dimensional non-woven structure of nano fibrils. XRD data found to be same pattern, such as same theta value and signal pattern. Both data implicated that BCs produced in the sludge and YG medium were same kind. To confirm that the BC produced can be used for the purpose of wound healing, BCs impregnated silver nanoparticles were prepared, and tested anti bacterial activity and water-holding capacity. Silver nanoparticle-impregnated bacterial cellulose showed an excellent moisture content compared to BC without silver nano particles. The freeze-dried silver nanoparticle-impregnated bacterial cellulose exhibited strong antimicrobial activity against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive).

In conclusion, mandarin wine sludge was sufficient to produce BC. It can be used as the BC producing medium. BC impregnated silver nanoparticles also can used as wound healing-aid.