비양나무 가지의 항산화, 미백, 항염 활성 성분 연구

Abstract

In this study anti-oxidative, whitening and anti-inflammatory activities were examined on the extract from Oreocnide fruticosa branches and their biologically active constituents were identified. From repeated fractionation procedures, seven compounds were isolated from Oreocnide fruticosa branches; friedelin (1), friedelanol (2),5,7-dihydroxychromone (3), methyl vanillate (4), catechin (5),epi-catechin (6) and scopoletin (7). The structures of these compounds were confirmed by comparing their spectroscopic data to those in the literature. All of these compounds (1-7) were isolated for the first time from O. fruticosa. In addition, the extract and isolated compounds were examined for anti-oxidative, whitening and anti-inflammatory activities. For the anti-oxidative activity test, the compounds 5 and 6 showed good DPPH radical scavenging activities with SC50 of 40.05 and 29.92 μM respectively, whose activities were comparable to a positive control vitamin C (SC50 46.23 μM). In addition, compounds 5, 6 and 7 showed good ABTS radical cation scavenging activity with SC50 of 7.56, 7.21, 64.04 μM respectively, showing comparable activity to vitamin C (SC50 31.32 μM) On the tyrosinase inhibition activity test, the compound 7 showed good inhibitory activity with IC50 111.80 μM, whose activities were more potent than a positive control arbutin (IC50 209.68 μM). For the anti-inflammatory activity test, the compound 7 showed inhibitory activity with 48.5% at 300 μM, without showing any cytotoxicity. Based on these results, O. fruticosa branches extract could be potentially applicable as cosmeceutical ingredient.