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Verbenone, a major compound of rosemary hydrosol inhibits melanogenesis and oxidative stress in B16F10 mouse melanoma cell

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Abstract
In the present study, we investigated the effect of verbenone, an active compound present in Rosmarinus officinalis L. hydrosol extract, on melanin synthesis and the molecular mechanism of melanogenesis in B16F10 mouse melanoma cells. The cellular melanin content and tyrosinase activity of the 2.5 mM verbenone-treated cells were significantly reduced below the levels of the kojic acid- treated cells, consistent with suppression of melanin biosynthesis by verbenone via inhibition of tyrosinase activity.Also verbenone inhibits the expression of melanogenesis-related proteins including MITF(Microphthalmia-associated transcription factor), tyrosinase and tyrosinase-related protein (TRP)s in α-MSH(a-melanocyte-stimulating hormone)-stimulated B16F10 cells. Moreover, the phospho-Akt was significantly enhanced but phospho-p38 MAPK was reduced and no effect phospho-ERK. Furthermore, the ROS generation submitted to an oxidative stress by H2O2treatment was completely inhibited by pre-treatment of verbenone,which is consistent with the increase in mRNA level of super oxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) in B16F10 cells. These data suggest that verbenone inhibits melanogenesis signaling by both inhibiting theexpression of melanogenesis protein and activating the Akt signal pathways-mediated suppression of MITF and its downstream signal pathway, including tyrosinase and TRP-1 and TRP-2. Therefore, verbenone would be a useful therapeutic agent for treating hyperpigmentation and can be applicable for cosmetics as skin-whitening agents.
Author(s)
전덕현
Issued Date
2014
Awarded Date
2014. 2
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000006698
Alternative Author(s)
Jeon, Deok Hyeon
Affiliation
제주대학교 대학원
Department
대학원 응용생명공학과
Advisor
김소미
Table Of Contents
1. INTRODUCTION 1
2. MATERIALS AND METHODS 5
2.1. Reagents
2.2. Cell culture
2.3. Gas Chromatography-Mass Spectrometry (GC-MS)
2.4.Cell viability
2.5.Melanin content
2.6.Intracellular tyrosinase activity
2.7.Mushroom tyrosinase activity
2.8.Cellular fraction and immunoblot analysis
2.9.RT-PCR analysis
2.10.Determination of ROS generation and Fluorescence microscope
2.11.Statistical analysis
3. RESULTS 12
3.1. Effect of the components of Rosmarinus officinalis L. hydrosol on melanin content
3.2. Effect of verbenone on cell viability in B16F10 cells
3.3. Effect of verbenone on melanin biosynthesis
3.4. Effect of verbenone on cellular or cell-free system of the tyrosinase activity
3.5. Effect of verbenone on thelevels melanogenesis protein in B16F10 cells
3.6. Effect of verbenone on the levels of melanogenic mRNA in B16F10 cells
3.7. Effect of verbenone on phosphorylation of p38, ERK and Akt in B16F10 cells
3.8. Effect of verbenone on intracellular ROS Generation
3.9. Effect of verbenone on the level of antioxidant enzyme mRNAin B16F10 cells
4. DISCUSSION 31
REFERENCES 33
ABSTRACT IN KOREAN 39
ACKNOWLEGEMENT
Degree
Master
Publisher
제주대학교 대학원
Citation
전덕현. (2014). Verbenone, a major compound of rosemary hydrosol inhibits melanogenesis and oxidative stress in B16F10 mouse melanoma cell
Appears in Collections:
General Graduate School > Biomaterials Science and Technology
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