Cytoprotective Effects of Americanin B against Oxidative Stress in Human Keratinocytes

Abstract

The aim of this study was to evaluate the cytoprotective effects of americanin B, a lignan compound, against hydrogen peroxide (H2O2)-induced cell damage. Americanin B decreased the level of DPPH radicals, superoxide anions, hydroxyl radicals and intracellular reactive oxygen species. Americanin B also attenuated DNA damage induced by H2O2 treatment, as shown by the inhibition of formation of comet tails indicative of DNA strand breakage, and prevented the oxidation of protein and peroxidation of lipid, as determined by protein carbonyls and 8-isoprostane. Furthermore, americanin B protected against H2O2-induced apoptotic cell death, as determined by a reduction in the numbers of apoptotic bodies stained with Hoechst 33342. These findings suggest that americanin B protects cells against oxidative damage by exerting antioxidant effects and inhibiting apoptosis.