소사나무 잎의 항산화, 미백,항염 및 항균 활성 성분 연구

Abstract

Carpinus turczaninowii Hance, a deciduous shrub growing in Jeju island, was investigated for anti-oxidative, skin whitening, anti-inflammatory and anti-bacterial activities. The dried C. turczaninowii leaves was extracted for 24 hours with 70 % ethanol. The obtained extract was successively partitioned into n-hexane (n-Hex), ethyl acetate (EtOAc), n-butanol (n-BuOH) and water (H2O) layers. For the anti-oxidation tests, the ethanol extract, EtOAc and n-BuOH layers showed good DPPH, ABTS+ radical and superoxide radical anion scavenging activities. The total phenolic contents for the extract and solvent layers (n-Hex, EtOAc, n-BuOH, H2O) were estimated as 279.3, 92.3, 447.3, 224.3 and 139.3 μg (GAE/1 mg) respectively. Moreover, when tested in B16F10 melanoma cells, EtOAc layer showed the good cellular melanogenesis inhibitory activity. In anti-inflammatory screenings, EtOAc layer showed the good nitric oxide production inhibitory activity in LPS-induced RAW 264.7 cell. Also on the screening of anti-bacterial activities, the extract and EtOAc layer showed the considerable inhibition for Staphylococcus epidermidis. Therefore, further phytochemical studies were conducted for the ethyl acetate layer, which led to isolation of four constituents such as ethyl gallate (1), quercitrin (2). afzelin (3), quercitrin-2''-O-gallate (4). As far as we know, all of the compounds except ethyl gallate (1), quercitrin-2''-O-gallate (4) were isolated for the first time from this plant. On screening of skin whitening activities, the compounds 1 and 4 showed considerable inhibition on the production of melanin contents for the B16F10 melanoma cell without showing cell toxicities. Based on these results, it is suggested that C. turczaninowii leaves extract could be potentially applicable in the cosmetical and/or pharmaceutical industries, especially as anti-oxidant, skin whitening, anti-inflammatory and anti-bacterial ingredient.