병개암나무 잎으로부터 항산화,항염 및 미백 활성 성분 규명

Abstract

Corylus hallaisanensis Nakai, a deciduous shrub growing in Jeju island, was investigated for skin whitening, anti-oxidative and anti-inflammatory activities. In skin whitening screenings, ethanol extract, n-Hex, EtOAc and n-BuOH layers showed melanin production inhibitory activity in α-MSH induced B16F10 murine melanoma cell. For the anti-oxidation tests, the ethanol extract, EtOAc, and n-BuOH layers showed good DPPH and ABTS+ radical scavenging activities. The total phenolic contents for the extract and solvent layers (n-Hex, EtOAc, n-BuOH, water) were estimated as 184, 64, 425, 347 and 83 μg GAE/1 mg respectively. In anti-inflammatory screenings, n-Hex and EtOAc layers showed nitric oxide production inhibitory activity in LPS-induced RAW 264.7 cell. Phytochemical studies were conducted for the EtOAc layer, which led to isolation of four constituents such as gallic acid (1), kaempferol 3-O-β -D-xylopyranoside (2), kaempferol 3-O-α-L-rhamnopyranoside (3) and apigenin (4). This study demonstrated the potential of Corylus hallaisanensis extract as the novel ingredients in functional cosmetics.