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Effects of the acute exposure oxytetracycline on the behavior and endocrine response in adult zebrafish

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Abstract
Zebrafish (Danio rerio) has been more widely used to study pharmacology.
Oxytectracycline (OTC) is a broad-spectrum antibiotic and works by interfering with the
ability of bacteria to produce essential proteins. The aim of this study was to identify the
effects of exposure to OTC on behavioral changes or endocrine response in zebrafish. The
behavioral effects of exposure to OTC (50, 100 or 200 mg/L) were characterized in several
novelty-based paradigms such as the novel tank or open field test in zebrafish. Moreover, to
investigate effects of exposure to OTC on endocrine response, we measured whole-body
cortisol level using cortisol ELISA assay kit. As results of novel tank test, duration in top and
immobile duration were significantly increased by the exposure to OTC in a concentrationdependent
manner (p < 0.05). On the other hand, distanced moved, highly mobile, velocity
and zone transition were significantly decreased by the exposure to OTC in a concentrationdependent
manner (p < 0.05). In addition, as results of open field test, the exposure to OTC
increased immobile duration significantly (p < 0.05). However, distance moved, mobile
duration and velocity were significantly decreased by the exposure to OTC in a
concentration-dependent manner (p < 0.05). Besides, the exposure to OTC elevated wholebody
cortisol levels in zebrafish. These results suggest that the exposure to OTC may induce
chemical stress in zebrafish.
Zebrafish는 약리학 연구에 널리 사용되는 실험 동물 중 하나이다.
Oxytetracycline은 넓은 항균 범위를 가지는 항생제로써 단백질 합성을 억제하여
정균 작용을 가진 항생제이다. 본 연구의 목적은 OTC에 노출이 되었을 때
zebrafish에게서 나타나는 행동변화와 내분비계의 변화를 관찰하였다. OTC(50, 100
or 200 mg/L)에 노출된 zebrafish의 행동변화를 확인하기 위해 novel tank test와 open
field test를 진행하였다. 또한 내분비계의 변화를 확인하기 위하여 whole-body
cortisol의 수치를 cortisol kit를 이용하여 측정하였다. Novel tank test 결과
상층부에서 머문 시간과 부동 시간에서 OTC를 약욕하였을 때 통계적으로
유의성이 있는 증가현상이 확인되었다. 반면에, 총 이동거리, 과행동 시간, 유영
속도 그리고 상하 변위에서 OTC를 약욕하였을 때 통계적으로 유의성 있는 감소
현상이 확인되었다. Open field test 결과 부동시간에서 OTC를 약욕 하였을 때
통계적으로 유의성 있는 증가 현상이 확인 되었다. 그러나 OTC를 약욕 하였을
때 총 이동거리, 움직인 시간, 유영 속도에서 통계적으로 유의성이 있는 감소
현상이 확인 되었다. Whole-body cortisol에서는 zebrafish에게 약욕을 하였을 때
증가하는 경향이 관찰되었다. 이러한 결과는 OTC가 zebrafish에게 화학적
스트레스로 작용한 것으로 사료된다.
Author(s)
고은성
Issued Date
2014
Awarded Date
2015. 2
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000007000
Alternative Author(s)
Eun Seong Ko
Department
대학원 해양생명과학과
Table Of Contents
1. Introduction 1
2. Materials & methods 2
2.1 Animal housing 2
2.2 Behavioral test 3
2.2.1 Novel tank test 3
2.2.2 Open field test 3
2.3 Measurement of whole-body cortisol 4
2.4 Statistical analysis 4
3. Result 5
3.1 Effect of OTC on novel tank test 5
3.2 Effect of OTC on open field test 7
3.3 Effect of OTC on whole-body cortisol level 9
4. Discussion 10
5. References 13
Abstract in Korean 17
Acknlowedgement 18
Degree
Master
Publisher
제주대학교 대학원
Citation
고은성. (2014). Effects of the acute exposure oxytetracycline on the behavior and endocrine response in adult zebrafish
Appears in Collections:
General Graduate School > Marine Life Sciences
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