솔잎과 경단구슬모자반으로부터 항산화, 미백 및 주름 개선 활성 성분 규명

Abstract

Phytochemical investigation of the 70 % ethanol extract from Pinus densiflora leaves led to isolation of four compounds ; catechin (1), shikimic acid (2), icariside E4 (3), (+)-isolariciresinol (4). In addition, the investigation of Sargassum muticum resulted in the isolation of three compounds ; mannitol (1), uracil (2), loliolide (3). For the total phenolic contents for the extract and solvent layers of P. densiflora were estimated as 114.6, 48.4, 188.4, 102.1, 108.4 μg (GAE/1 mg) respectively. And the total polyphenolic contents for the extract and solvent layers of S. muticum were estimated as 20.9, 23.4, 24.6, 20.9, 18.4 μg (GAE/1 mg) respectively. The total flavonoids contents for the extract and solvent layers of P. densiflora were estimated as 16.8, 40.1, 82.6, 10.9, 10.9 μg (quercetin/1 mg) respectively. In case of S. muticum, the extract and solvent layers were estimated as 14.3, 10.9, 38.4, 8.4, 8.4 μg (quercetin/1 mg) respectively. The anti-oxidation tests, the ethanol extract, EtOAc, BuOH and H2O fractions of P. densiflora showed good DPPH and ABTS+ radical scavenging activities. However extract and solvent layers of S. muticum showed not good activities. On the elastase inhibition studies, the extract and solvent layers of P. densiflora showed good elastase inhibition activities. But S. muticum did not show good elastase inhibition activities. On the melanogenesis inhibition activity test using B16F10 cell, n-Hex layer of P. densiflora and n-Hex, EtOAc layer of S. muticum showed good melanogenesis inhibition activities. Based on these results, it is suggested that Pinus densiflora leaves and Sargassum muticum extract could be potentially applicable in the cosmetical and/or pharmaceutical industries, especially as anti-oxidant and anti-aging, skin whitening ingredients.