RAW 264.7 cell에서 까마귀쪽나무(Litsea japonica) 종자 초임계 추출물의 염증 억제 효과

Abstract

This study describes a preliminary evaluation of the anti-inflammatory activity of Litsea japonica seed. The supercritical extraction of L. japonica seed (LSSE) was obtained using supercritical carbon dioxide, and supercritical carbon dioxide extract (9.48% at 300 bar, 60℃) at concentration of 10 mg/mL. The LSSE dose-dependently inhibited the production of inflammatory markers [nitric oxide (NO), inducible nitric oxide synthase (iNOS), prostaglandin E2(PGE2), andcyclooxygenase-2(COX-2)] and pro-inflammatory cytokines [tumornecrosisfactor-α (TNF-α), interleukin (IL)-1β, and IL-6] induced by LPS treatment. To further elucidate the mechanism of this inhibitory effect of LSSE, we studied LPS-induced nuclear factor-κB (NF-κB) activation and mitogen-activated protein kinases (MAPKs) phosphorylation. LSSE inhibited the phosphorylation IκB-α, NF-κB and MAPKs in a dose dependent manner. These results suggest that the anti-inflammatory activity of LSSE results from its modulation of pro-inflammatory cytokines and mediators via the suppression of NF-κB activation and MAPK phosphorylation. This research was supported by High Value-added Food Technology Development Program, Ministry of Agriculture, Food and Rural Affairs(SRAA).