제주달구지풀의 삽목증식 및 육묘환경 연구

Abstract

In the present study, we aimed to establish measures for preservation and restoration of genetic resources via cuttage propagation and seedling environment investigations for Trifolium lupinaster f. alpinus (Nakai) M.Park thereby providing basic research data for industrialization thereof. The average temperature of natural habitat for Trifolium lupinaster f. alpinus (Nakai) M.Park, Youngsil-area, was 12.3°C in between March through October and it was found to be highest in July as 19.0°C; the average humidity of Youngsil-area was 78% and it was highest in June as 98.8%. Regarding vegetation, Arundinella hirta var. ciliata Koidz., Festuca ovina, Cirsium rhinoceros, Potentilla dickinsii Franch. & Sav., and Thymus quinquecostatus Celak. were found in the quadrat. The average temperature of vinyl moist chambers between April through June was 22.3°C in which the cuttage experiments of Trifolium lupinaster f. alpinus (Nakai) M.Park were implemented while the average humidity was reported as 93.3%. During the same period, the average temperature and humidity of general practice green houses were 19.3°C and 70.3%, respectively. The average temperature and humidity of general practice green houses were 24°C and 81.0% in which the early growth experiments were performed. When performing cuttage, there was no significant difference in the rooting rate of Trifolium lupinaster f. alpinus (Nakai) M.Park between vinyl moist chambers and green houses yet the length and numbers of roots were found to be more favorable in the green houses. When it comes to effects of containers on the rooting rate, no significant difference was noted as all groups had 100% rooting rate. There were however, favorable results found with regards to root length as well as diameter in the pot and cultivating box. Effects of pretreatments on rooting characteristics were also investigated; as results, all pretreatments (i.e., control group, lime water treated group, potassium permanganate treated group, and rooting promoter treated group (RootoneTM)) represented higher than 90% of rooting rate whereas the group with silver nitrate showed poor rooting rate compared to other groups; lastly, rooting properties, such as length and numbers of roots, were more favorable in the groups treated with potassium permanganate or the rooting promoter, RootoneTM. When seedling Trifolium lupinaster f. alpinus (Nakai) M.Park in different containers, lengths of aerial part as well as of roots were longest in the cultivating boxes followed by pots, and trays. With varying amount of applied nitrogen, as a fertilizer, the survival rate of Trifolium lupinaster f. alpinus (Nakai) M.Park tends to be decreasing with increasing amount of nitrogen yet it was not statistically significant. The length of aerial part was increased approximately 46% compared to the control group when 15kg/10a of nitrogen was applied. Taken altogether, it is considered that Trifolium lupinaster f. alpinus (Nakai) M.Park is a well-rooted species hence propagation of this plant is thought to be relatively easy. Given that it belongs to the leguminous plant, its industrial potential might be realistic (high).