해산어류 사료 내 가소화단백질/에너지비 평가 및 지질 이용성에 관한 연구

Abstract

Effect of dietary protein and lipid levels on growth, feed utilization and innate immunity of red seabream (Pagrus major) A 3 × 3 factorial study was conducted to investigate the effect of dietary protein and lipid levels on growth, feed utilization and innate immunity of red seabream. Nine diets containing three protein levels (42, 46 and 50% crude protein) and three lipid levels (10, 12 and 18% crude lipid) were formulated. Triplicate groups of red seabream were fed the experimental diets to apparent satiation (5-6 times a day, from 08:00 to 18:00 h with 2 h intervals) for 10 weeks. At the end of feeding trial, weight gain and specific growth rate of fish fed P46L14 (46%CP+14%CL), P50L10 (50%CP+10%CL) and P50L14 (50%CP+14%CL) were significantly (P<0.05) higher than those of the fish fed the P42L18 (42%CP+18%CL). Myeloperoxidase activity in the group fed the P50L14 (50%CP+14%CL) was significantly higher than the groups fed the P42L10 (42%CP+10%CL) and P50L18 (50%CP+18%CL). The fish fed P46L14 (46%CP+14%CL) and P46L18 (46%CP+18%CL) showed significantly higher SOD activity than the fish fed P46L10 (46%CP+10%CL), P50L10 (50%CP+10%CL) and P50L18 (50%CP+18%CL). The results of this study showed that the red seabream grows well when fed diets containing 46% dietary protein with 14% lipid at dietary DP/E ratio 27.4 g/MJ. Effects of dietary supplementation of emulsifiers on growth performance, feed utilization and innate immunity of red seabream (Pagrus major) Five experimental diets were formulated to contain HE(hydrophilic emulsifier) and LE(lipophilic emulsifier) at two different levels of 0.15 and 0.3%. Triplicate groups of red seabream were fed the experimental diets to apparent satiation (twice daily, 08:00 and 18:00 h) for 10 weeks. At the end of the feeding trials, weight gain, specific growth rate, feed conversion ratio, protein efficiency ratio, feed intake and survival of fish were not significantly affected by the supplementation of HE and LE. Lysozyme activity was significantly increased when the fish were fed HE 0.3% diet in comparison that of fish fed another diet. No beneficial effects were found on growth performances and feed utilization by the supplementation of HE or LE in diets for red seabream while 0.3% HE can improvenon-specific immune response. Use of diglycerides to replace fish oil in diets for olive flounder(Paralichthys olivaceus) Six experimental diets were formulated to replace50% or 100%fishoilby differentdiglycerol (low purity1,3-lauryl glycerol, DGLL high purity1,3-lauryl glycerol, DGLH low purity1,3-palmityl glycerol, DGPLhigh purity1,3-palmityl glycerolDGPH; 50% low purity 1,3-lauryl glycerol + 50% low purity 1,3-palmityl glycerol, DGLP)at50%and100%.Triplicate groups of olive flounderwere fed the experimental diets to apparent satiation (twice daily, 08:00 and 18:00 h) for 12weeks.No significant difference was observed in growth performance and feed utilization between the control and replacement groups. Replacement of dietary fish oil up to 100% by DGLP is possible in olive flounder with no reduction in growth performance and feed utilization. Effect of replacement of dietary fish oil by diglycerides on growth, feed utilization and fatty acid composition in olive flounder (Paralichthys olivaceus) Six experimental diets were formulated to replace fishoilby two differentdiglycerol (LPP(2) and LPP(3))at 0.5%, 1.0% and 1.5% (designated asLPP(2)0.5, LPP(2)1.0, LPP(2)1.5and LPP(3)1.0)Triplicate groups of olive flounderwere fed the experimental diets to apparent satiation (twice daily, 08:00 and 18:00 h) for 8 weeks.No significant difference was observed in growth performance and feed utilization between the control and replacement groups. Over 30% replacement of fish oilby LPP seems tobe possible in olive flounder.