까마귀쪽나무(Litsea japonica)종자로부터 항염 및 미백 활성 성분 연구

Abstract

Litsea japonica, a evergreen plant growing in Jeju island, was investigated for anti-inflammatory and skin whitening activities. The dried L. japonica seeds were extracted for 72 hours with 100% methylene chloride. The obtained extract was successively partitioned into n-hexane (n-hex), methylene chloride (CH2Cl2), 20% MeOH layers. Repeated column chromatography on celite and normal-phase silica gel resulted in the isolation of seven compounds; glyceride (compound 1, 2, 3, 4), litsenolide B1 (compound 5), litsenolide A1 (compound 6), and litseakolide D2 (compound 7). In anti-inflammatory screenings, extract and CH2Cl2 layer, compounds 5 and 6 inhibited nitric oxide production in LPS-stimulated RAW 264.7 cell. Moreover, on screening of skin whitening activities, the compounds 5 and 6 showed considerable inhibition on the production of melanin contents in the B16F10 melanoma cell without showing cytotoxicity. This study demonstrated the potential of Litsea japonica seed extract as the novel ingredients in functional cosmetics.