동백동산 양치식물의 염기서열에 의한 분류

Abstract

In this research, the Pteridophyta recorded in Seupjibohoguyeok Jeongmiljosa Dongbaek Dongsan Park Section has been reexamined and reclassified according to the base sequence, and also the effectiveness of primer presented based on this classification has been tested. There are 26 species of Pteridophyta which are presented in countrywide wetland research-Dongbaek Dongsan Park Section. 22 species have been collected at Dongbaek Dongsan Park and other Pteridophytes which were not found there Dryopteris varia(L.)Kuntze(족제비고사리), Neocheiropteris ensata(Thunb.)Ching(밤일엽), Botrych ium virginianum(L.)Sw.(늦고사리삼), Coniogramme japonica(Thunb.)Diels(가지고비고사 리), have been collected in another spontaneous land. The universal primer used in this research is the primer c and d of trnL-L region of chloroplast DNA and the primer e and f of trnL-F region. For additional primer, primer 1FN and 1361R of rbcL region and fern1 and F of trnL-F region have been used. As a result of analyzing base sequence except Thelypteris acuminata(Houtt.) Morton(별고사리), has been identified as Lemaphyllum microphyllum C.presl var. obovatum.(긴콩짜개덩굴) Base Sequence of all the other Pteridophyta was the same, Cyrtomium falcatum J. Sm.(쇠고비) recorded 93% while Mankyua chejuense B.Y.Sun,M.H. Kim&C.H.Kim(제주고사리삼) recorded 94% of homogeny rate. Except Onychium japoicum (Thunb.)Kunze(선바위고사리), Pyrrosia lingua (Thunb.)Farw.(석위), Lemmaphyllum microphyllum C. Presl(콩짜개덩굴), except Thelypteris acuminata(Houtt.)C.V.Morton(별고사리) among 26 species of Pteridophyta, universal primer c and d, e and f formed base fragments and suggested 85% of identification rate. When primer 1FN and 1361R, fern 1 and F are used additionally, all 26 species of Pteridophyta were identifiable except except Thelypteris acuminate C.V.(Houtt.) (별고사리), recording 96% of identification rate. Recently, researches on classification and system of pteridophyta have been conducted based on base sequence. By means of base sequence analysis, independent gametophyte, Trichomanes intricatum, proved to have same base sequence as one of pteridophyta called Crepidomanes schrnidtianum(두메괴불이끼). As for the system, Lycopodiaceae and Isoetes which were included in existing pteridophyta has been Lycophytes due to the grafting of base sequence. The research on base sequence of the pteridophyta of South Korea has been limited to specific species; this research is to present universal primer which can identify various species of pteridophyta.