수박 지상부 추출물의 항염 및 미백 활성 성분 연구

Abstract

In this study, anti-inflammatory and skin-whitening activities were examined on the extract from Citrullus lanatus stems and leaves. The Citrullus lanatus stems and leaves were extracted three times for 24h each with 70% ethanol. The ethanol extract was then suspended in distilled water and partitioned successively with n-hexane, ethyl acetate, n-butanol and water. Repeated column chromatography on normal-phase silica gel and Sephadex LH-20 resulted in the isolation of three compounds, α-linolenic acid (1), sigmast-7-en-O-β-D-glucopyranoside (2) and 1-feruloyl-β-D-glucopyranoside (3). The elucidation of the chemical structure of the compounds 1-3 were accomplished using spectroscopic data including 1D and 2D NMR spectra, and by the comparison of their data to the literature values. In anti-inflammatory screenings, n-hexane, ethyl acetate layers and compounds 1, 3 showed nitric oxide production inhibitory activity in LPS-induced RAW 264.7 cell. Moreover, on screening of skin whitening activities, the n-hexane, ethyl acetate layers and compounds 1, 3 showed considerable inhibition on the production of melanin contents in α-MSH induced B16F10 murine melanoma cell without showing cytotoxicity. Based on these reults, Citrullus lanatus stems and leaves extract were suggested for use as the novel ingredients in functional cosmetics.