섬오가피나무 줄기로부터 항산화, 미백, 항염 및 항균 활성 성분 규명

Abstract

In this study, anti-oxidative, whitening, anti-inflammatory and anti-bacterial activities were screened on the extracts from the stems of Acanthopanax koreanum and their active constituents were isolated and identified. A. koreanum was investigated to identify the constituents possessing anti-oxidative, whitening, anti-inflammatory and anti-bacterial activities. The dried A. koreanum (1 kg) was extracted three times for 24 hours using 70 % EtOH. The obtained extract was successively partitioned into n-Hex, EtOAc, n-BuOH and H2O fractions. On the anti-oxidative test, EtOAc fraction had good DPPH radical scavenging activity. Moreover, n-Hex and EtOAc fractions had potent whitening, anti-inflammatory and anti-bacterial activities. Therefore, further phytochemical studies were conducted for the n-Hex and EtOAc fractions, which led to isolation of eight constituents such as 3,4-dihydroxybenzoic acid (1), 3,4-dihydroxybenzoic acid methyl ester (2), caffeic acid (3), chlorogenic acid methyl ester (4), coriolic acid (5), 16α-hydroxy-17-isovaleroyl-oxy-ent-kauran-19-oic acid (6), 16α-hydro-17-isovaleroyloxy-ent-kauran-19-oic acid (7) and kaurenoic acid (8). As far as we know, compounds 1-5 were isolated for the first time from this plant. In order to study the skin-related properties for the isolated compounds, bioactivity screenings on anti-oxidative, whitening, anti-inflammatory and antibacterial tests were conducted. On anti-oxidative test, the compounds 1, 2, 3 and 4 showed good DPPH radical scavenging activity. On the skin-whitening activities, the compounds 6, 7 and 8 inhibited the cellular melanogenesis and tyrosinase activities dosedependently in α-MSH-stimulated B16F10 cells. In anti-inflammatory screenings, the compounds 2, 4, 6 and 7 significantly inhibited NO, TNF-α and IL-6 production dose-dependently in LPS-stimulated RAW 264.7 cells. On the anti-bacterial activities, the compounds 2, 3, 5, 6 and 8 showed the moderate anti-bacterial activities against bacteria causing skin infection. Based on these results, A. koreanum stem's n-Hex, EtOAc fractions and/or isolated compounds could be potentially applicable as cosmeceutical ingredients.