국내에서 구조된 어린 올빼미의 종과 성감별 및 성장에 따른 혈액화학치의 변동

Abstract

Most of rescued young tawny owls are missing individuals in Spring and it is very important to morphologically distinguish another little avian family entity like Strigidae. There are no data of the normal reference of blood and plasma biochemistry values for assessing health status during tawny owls growth process. In this study, the rescued young tawny owls on May 2014, 2015 are identified species and sex using molecular biological methods and five objects of them are done a blood test and the measurement of body weight per a month until returning of nature on December 2014. There are three types of samples such as 3 objects of young tawny owls from Conservation Genome Resource Bank for Korean Wildlife, 8 objects in 2014 and 1 object in 2015 of the rescued little Tawny owls from Chungnam Wild Animal Rescue Center to be identified conspecific and done sex identification. Species identification was carried out sequencing analysis of mitochondrial genetic code for Cytochrome c oxidase 1 (CO1) and sex identification was implemented using the amplification of the Chromo– helicase-DNA binding(CHD) gene, and resulted in confirming W(900-1,000 bp) and Z(500-600 bp), and found out 3 males(ZZ) and 6 females(ZW) among 9 individuals. Tendency of first increase and then decrease of ALPK and Ca after basic blood test and plasma biochemistry examination was observed, and in general PCV, TP, ALB, GLOB were increased and LDH, CK were decreased but AST, GLU, URIC were confirmed to be free from changing during examination. The basic data of healthy individuals through the change of measuring body weight and physical measurement were identified. These results indicate that the species and the sex identification of rescued young tawny owls, and the normal reference of blood and plasma biochemistry values during growth progress will be useful in the field of clinical evaluation of owl's species save, treatment and protection management as a basic data of species conservation and research.