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제주도 양식 넙치(Paralichthys olivaceus)를 대상으로 한 바이러스성 질병 모니터링

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Alternative Title
Monitoring of viral diseases in cultured olive flounder, Paralichthys olivaceus in Jeju
Abstract
Viruses are known as serious pathogens, which can cause severe disease sea
fish diseases, especially red seabream iridovirus (RSIV), viral haemorrhagic septicaemia
virus (VHSV), viral nervous necrosis virus (VNNV), marine birnavirus (MABV)
and hirame rhabdovirus (HRV). VHSV has been a serious viral disease that infects
the olive flounder in South Korea. Clinical signs of VHSV infection are skin darkening,
abdominal distension and haemorrhages. Outbreaks of fish iridovirus disease was
first reported from red seabream, Pagrus major farms in Japan. Recently, iridovirus
infection have occurred frequently from olive flounder farms in South Korea. VNNV
is a worldwide viral disease affecting several species of cultured sea fish such as
olive flounder in South Korea. MABV is also causes serious problems in the olive
flounder farming industry in Japan. The infectious marine birnavirus are well
known fish pathogens in Asian countries such as Korea and China (olive flounder
farm). The initial case of HRV was reported from olive flounder farms in Japan.
Since then, HRV infections with severe mortalities have occurred at olive flounder
farms in South Korea.
In this study, disease surveillance was performed to monitor the prevalence of
viral diseases in olive flounder from 2014 to 2015. The fish samples were collected
from 60 different farms of Jeju in April, May, September, November and December
2014. In 2015, the fish samples were collected in March (60 farms), May (55 farms),
July (52 farms) and October (53 farms) from different farms. RT-PCR or PCR results
showed that VHSV were detected in 7 farms (2014-5, 2015-2). In addition, the
suspected of viral disease were detected by PCR with each specific primers.
RT-PCR or PCR results showed that VHSV were detected in 8 farms (2014-5,
2015-3), but other viral diseases have not been detected in any farms.
The sequences of the nucleocapsid protein (N) gene and glycoprotein (G) gene
of 15 VHSV isolates were successfully amplified and sequenced. Phylogenetic analysis
was performed using the VHSV sequences reported here together comparison
with the nucleotide sequences available from the GenBank database. Phylogenetic
analysis indicated that most of Korea VHSV belong to the genotype Ⅳa and closely
related to the strains from Japan and China.
Author(s)
박현경
Issued Date
2015
Awarded Date
2016. 2
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000007467
Alternative Author(s)
Park, Hyun Kyung
Department
대학원 해양생명과학과
Advisor
정준범
Table Of Contents
Ⅰ. 서 론 1
Ⅱ. 재료 및 방법 5
1. 실험어 5
2. PCR 및 sequencing 5
2.1. Primers 제작 5
2.2. DNA 추출 7
2.3. RNA 추출 및 cDNA 합성 7
2.4. PCR 및 sequencing 8
3. 계통학적 분석 8
4. 바이러스 배양 13
Ⅲ. 결과 14
1. PCR 결과 14
1.1. 2014년도 바이러스성 질병 모니터링 14
1.2. 2015년도 바이러스성 질병 모니터링 16
1.3. 기타 바이러스성 질병 검사 18
2. 계통분류학적 분석 및 유전자 상동성 비교 20
2.1. VHSV N gene 20
2.2. VHSV G gene 22
3. 바이러스 배양 24
Ⅳ. 고찰 25
Ⅴ. 요약 30
Ⅵ. 참고문헌 32
Ⅶ. 감사의 글 39
Degree
Master
Publisher
제주대학교 대학원
Citation
박현경. (2015). 제주도 양식 넙치(Paralichthys olivaceus)를 대상으로 한 바이러스성 질병 모니터링
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General Graduate School > Marine Life Sciences
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