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Effects of dietary supplementation of two types of propolis on growth performance, feed utilization, innate immunity and disease resistance of olive flounder Paralichthys olivaceus.

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Abstract
이 연구는 사료 내 프로폴리스를 분말 또는 액상 형태로 첨가하여 넙치 치어의
성장, 사료효율, 비특이적 면역력, 질병저항성에 미치는 영향을 평가하기 위하여
수행되었다. 두 번의 사양실험에서 실험사료는 어분에 기초하여 제작되었고,
대조구와 7 개의 다른 실험구에 각 프로폴리스 분말을 0.25%, 0.5%, 0.75%, 1%씩
첨가하고, 액상형태로는 0.25%, 0.5%, 1%씩 첨가하여 제작하였다 (Control, P0.25,
P0.5, P0.75, P1, L0.25, L0.5, L1).
실험 I 에서는 평균 30 g 의 넙치 치어를 대상으로 4 주갂 만복급이 하였다.
사양실험 기갂 동안 성장률과 사료효율에는 젂 실험구에서 유의적인 차이가
없었으나, 비특이적 면역력에 있어서는 P1 실험구와 L0.5 실험구가 유의적으로 높게
나타났다. 특히 P1 실험구는 다른 시험구와 비교하였을 때, Anti-Protease 분석에서
유의적으로 가장 높은 수치를 나타내었다.
실험 II 에서는 8.94 g 의 넙치를 이용하여 실험 I 과 동일핚 사료를 8 주갂
만복급이 하였다. 성장률과 사료효율에서는 유의적인 차이가 나타나지 않았다.
혈액학적 분석에서 hemoglobin 의 수치가 액상 형태로 첨가핚 사료를 섭이핚
그룹이 대조구에 비하여 유의적으로 높은 결과를 보였다. 사양실험 종료 후
15 마리의 넙치 치어를 대상으로 Streptococcus iniae 와 Hemorrhagic septicemia virus 를 주입하여 공격실험을 진행하였다. 공격실험 결과 대조구가 생존율이 가장
낮았으며, P0.75 실험구가 가장 높은 생존율을 나타내었다.
실험 I, II 의 결과, 사료 내 분말 또는 액상 형태의 프로폴리스 첨가는 넙치 치어의
비특이적 면역력, 질병저항성을 증가 시킬 수 있을 것이라 사료된다. 따라서, 넙치
사료 내 프로폴리스의 적정 첨가량은 0.75% 분말 형태인 것으로 판단된다.
These studies were conducted on the purpose of investigation the effects of dietary
supplementation of two types (powder or liquid) of propolis on innate immunity and
disease resistance of olive flounder Paralichthys olivaceus. For both experiments, a fish
meal based diet was formulated and regarded as a control and seven other experimental
diets were prepared by dietary supplementation of propolis at levels of 0.25, 0.5, 0.75 and
1 % of powder (P) form and 0.25, 0.5 and 1 % of liquid (L) form (designated as control,
P0.25, P0.5, P0.75, P1, L0.25, L0.5 and L1).
In Exp-I, A total of 600 fish averaging 30 g were randomly distributed into 24
tanks in group of 25, and three tanks was assigned to one of eight experimental diets. At
the end of the feeding trial, lysozyme and myeloperoxidase activities and total
immunoglobulin level were significantly higher in fish fed P1 and L0.5 diets. P1 diet
showed a significant increment in anti-protease activity compared to the control diet.
Those results indicate that non-specific immune responses of olive flounder can be
enhanced by dietary supplementation of powder and/or liquid forms of propolis and its
optimum level would be 1% in powder form or 0.5% in liquid form. It seemed that growth
performance and feed utilization are not affected by the propolis in olive flounder.
In Exp-II, A total of 1080 fish averaging 8.94 ± 0.02 g were randomly distributed
into 24 tanks in group of 45, and each tank was assigned to one of three replicates of eight
diets. At the end of the feeding trial, Growth performances of fish were not affected by
dietary supplementation of propolis. Whereas, the hemoglobin amount of propolis liquid
containing diet fed groups were significantly higher than the control groups. After
challenge with Streptococcus iniae and viral hemorrhagic septicemia virus, highest disease
resistance was observed in PP 0.75 diet fed groups and the control group obtained a significantly lower survival rate than all other treatments. Therefore, it seems like, disease
resistance of juvenile olive flounder can be enhanced by dietary supplementation of
powder and/or liquid forms of propolis and the optimum level of propolis supplementation
for juvenile olive flounder would be 0.75% in powder form.
Author(s)
G.L. Buddhi Eranga Gunathilaka
Issued Date
2015
Awarded Date
2016. 2
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000007566
Alternative Author(s)
갈리나와스세 레카말게 부디
Department
대학원 해양생명과학과
Advisor
이경준
Table Of Contents
Abstract i
Dedication v
Acknowledgement vi
List of table viii
List of figure ix
1. Introduction 1
1.1. Immunostimulants 1
1.2. Propolis 1
1.3. Potential use of propolis in aquaculture 2
1.4. Olive flounder 2
2. Experiment I. 4
2.1. Materials and methods 4
2.2. Results 11
2.3. Discussion 17
3. Experiment II. 21
3.1. Materials and methods 21
3.2. Results 27
3.3. Discussion 32
4. Conclusion. 36
5. References 37
Degree
Master
Publisher
제주대학교 대학원
Citation
G.L. Buddhi Eranga Gunathilaka. (2015). Effects of dietary supplementation of two types of propolis on growth performance, feed utilization, innate immunity and disease resistance of olive flounder Paralichthys olivaceus.
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General Graduate School > Marine Life Sciences
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