해마 (Hippocampus abdominalis) 효소가수분해물의 항산화 활성 및 생리활성 펩타이드의 분리

Abstract

Seahorse, Hippocampus abdominalis (H. abdominalis) known as a marine teleost fish, has been used as one of the most famous and expensive materials of traditional Chinese medicine due to its beneficial biological activities. However, its use has been limited by its high cost despite its beneficial biological activities and there are few reports on its biological activity and active components. Therefore, we investigated the antioxidant activity of H. abdominalis and its active compounds. First of all, to improve its extraction yields, we prepared the water-enzymatic hydrolysates by performing the water and enzymatic extraction techniques with the seven food grade enzymes (Trypsin, Pepsin, Neutrase, Protamex, Alcalase, Kojizyme, and Flavourzyme) in consecutive order. The enzymatic extracts showed the higher extraction yield than that of the only water extract. Among them, the water-Pepsin hydrolysate showed the higher DPPH, hydroxyl and alkyl radicals, and hydrogen peroxide scavenging activities. Also, it protected against oxidative stress caused by the treatment of AAPH. To identity its active components, we fractionated the > 5 KDa and < 5 KDa molecular weight fractions from the Water-Pepsin hydrolysates and confirmed the superior antioxidant activity of < 5 kDa frction. The < 5 KDa fraction, a low molecular weight fraction was applied to Sephadex G-25 gel filtration chromatography and we identified that the isolated most low molecular weight fraction led to the highest alkyl radical scavenging activity. Results of examining amino acid composition of the active peptide, it was possible to confirm the presence of peptides consisting with mainly many tryptophan and glycine. In conclusion, in this study suggests that the seahorse water-enzymatic hydrolysates prepared by the useful water-extraction techniques has the antioxidant activity and might be an useful resource of marine origin as a novel material for healthy functional foods and cosmetics.