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Biomass and lipid accumulation of oceanic microalga Chlorella sp. in peritoneal dialysis waste fluid supplemented mixotrophic culture

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Abstract
Interest in the use of microalgal biodiesel production using nutrients from wastewater has been increasing due to the cost-effectiveness of such processes. The aim of this study was to evaluate the feasibility of using peritoneal dialysis waste fluid (PDWF) from renal failure patient as an additive to the algal culture medium used for biodiesel production. The obtained PDWF contained large amounts of glucose (9.78 g L-1), nitrogen, phosphorus, and iron, along with various inorganic elements. Supplementation of the culture medium of Chlorella sp. CKC2 with 0–1000 mg L-1 of dried-PDWF (DPDWF) resulted in concentration-dependent increase in growth, with the maximum increase in biomass (1.3-fold) at 1000 mg L-1, compared to culture not supplemented with DPDWF (0 mg L-1). The lyophilized biomass exhibited the maximum amount of increased total lipid content (1.31-fold, wt%) at 1000 mg L-1, compared to the control. Differences in the fatty acid composition of supplemented and un-supplemented cultures induced no significant changes in the biodiesel properties. The results suggest that mixotrophic cultivation using readily available DPDWF as a carbon source, could provide a significantly lower-cost method for algal biodiesel production, and at the same time, provide a neat solution for disposal of dialysis-derived wastewater.
Author(s)
이치헌
Issued Date
2017
Awarded Date
2017. 2
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000007956
Alternative Author(s)
Chi-Heon Lee
Department
대학원 해양생명과학과
Advisor
여인규
Table Of Contents
ABSTRACT ABSTRACT ⅰ
CONTENTS ⅱ
LIST OF TABLES ⅳ
LIST OF FIGURES ⅴ
Ⅰ. INTRODUCTION 1
Ⅱ. MATERIALS AND METHODS 3
1. Algal source and purification 3
2. Preparation of peritoneal dialysis waste fluid (PDWF) 3
3. Lab-scale growth test and biomass determination 5
4. Analytical methods . 5
4.1. Inductively coupled plasma mass spectrometer (ICP-MS) analysis of PDWF . 5
4.2. Monosaccharide composition, total nitrogen (TN) and total phosphorus (TP) determination 6
4.3. Total carbohydrate determination . 6
4.4. Total lipid extraction and fatty acid methyl esters (FAMEs) analysis 7
5. Statistical analysis . 8
Ⅲ. RESULTS AND DISCUSSION . 9
1. Monosaccharide composition and amounts of total nitrogen (TN) and total phosphorus (TP) 9
2. Inorganic element composition of peritoneal dialysis waste fluid (PDWF) . 12
3. Effect of different concentrations of PDWF on the biomass production of Chlorella sp. 14
4. Growth and biomass production of Chlorella sp. under DPDWF supplemented mixotrophic culture and uptake of glucose . 17
5. Effect of DPDWF on the photosynthetic pigment accumulation of Chlorella sp. 18
6. Effect of DPDWF on the lipid accumulation of Chlorella sp. 20
7. Effect of DPDWF on the fatty acid methyl ester (FAME) composition of Chlorella sp. 22
8. Feasibility of PDWF . 24
Ⅳ. CONCLUSION . 25
Ⅴ. REFERENCES . 26
ACKNOWLEDGEMENT 33
Degree
Master
Publisher
제주대학교 일반대학원
Citation
이치헌. (2017). Biomass and lipid accumulation of oceanic microalga Chlorella sp. in peritoneal dialysis waste fluid supplemented mixotrophic culture
Appears in Collections:
General Graduate School > Marine Life Sciences
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