콘택트렌즈 다목적 용액의 항균활성 물질 연구

Abstract

Naturally derived compounds(trans-chalcone, quercetin, tannic acid), extracts of native plants in Jeju region(Oenothera laciniate Hill, Phyllanthus urinaria L.), and extracts of marine plants(Saragassum muticum (Yendo) Fensholt) were examined for the antibacterial activities against the contact lens related strains (Staphylococcus aureus, Pseudomonas aeruginosa, Serratia marecescens, Enterobacter cloacae) and were evaluated for the possibility of its usage as a disinfectant in the contact lens multipurpose solution. Trans-chalcone, quercetin, and tannic acid were found to have antimicrobial activity against gram-positive bacteria, S. aureus, and trans-chalcone showed the best inhibitory activity amongst the three (MIC 62.5 μg/mL, IC90 15.2 μg/mL, IC50 8.9 μg/mL). The EtOH extract of Phyllanthus urinaria L. was found to have antimicrobial activity against both gram-positive(S. aureus) and gram-negative(E. cloacae) bacteria. MIC was found to be less active against S. aureus, unable to find MIC under 1,000 μg/mL but its antimicrobial activity for E. colacae was found to be high (MIC 3.9 μg/mL, IC90 2.4 μg/mL, IC50 2.0 μg/mL >). The hot water extract of Phyllanthus urinaria L. showed the antimicrobial activity against S. marecescens and the hot water extract of Saragassum muticum showed the antimicrobial activity against P. aeruginosa. Trans-chalcone, which has the highest antimicrobial activity against S. aureus with its high rate of revelation among contact lens-inducing strains, was added to the F brand product, which has shown to be weak in activity, to observe the changes in the sterilizing power against S. aureus and the changes in physical properties of contact lens(water content, refractive index, luminous transmittance, tensile strength, contact angle). As the result, the bactericidal activity against S. aureus, which is a contact lens related strain, was shown to be improved and the changes in the physical properties of contact lens was also found to be the same or smaller when compared with the control group. Therefore, these results suggest that trans-chalcone may be applicable as a disinfectant for multipurpose solutions. In addition, opticians educating contact lens care were examined for the importance of contact lens duties and the awareness of the need for education; and the female university students who are the main consumers of contact lenses were investigated for the contact lens wear management status and eye health awareness. It has been found that for opticians, female opticians than male opticians, ophthalmic clinic employees than optical shop employees, CEO or managers than average employees are more aware of the importance of the job duties and educational needs. The results of the survey on the contact lens wearing status and eye health perception of female college students showed that contact wearing rate was increased due to cosmetic reasons and the feeling of dryness was the highest inconvenience caused by wearing contact lenses. The most common cause of discomfort was the contact lenses wear for a long period of time. The awareness of eye health knowledge related to wearing contact lenses was found relatively high(69.8%), but the awareness of contact lens related items such as contact lens storage containers was found to be the lowest. Among the opticians who are responsible for the management and education of contact lenses, the awareness of the importance of contact lens duties and educational needs were exhibited differently according to gender, working occupation, position in the industry, and the tasks they are mainly responsible for. In addition, users seemed to have a general understanding of the eye health knowledge related to contact lenses that can reduce the inconvenience caused by wearing contact lenses, but the understanding of contact lens accessories management was found to be low. The satisfaction rate with wearing contact lenses is thought to be improved with an appropriate education and continuous follow-up care are provided with a consideration for the wearer's working environment, daily wear time, the main discomfort the wearer complains of, the types and materials of lens used.