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붉바리(Epinephelus akaara)의 성숙 개시와 성 특성

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Alternative Title
Puberty and sex characteristic in red spotted grouper, Epinephelus akaara
Abstract
It takes quite a long time for the grouper to spawn. In the case of red spotted grouper (Epinephelus akaara), at least three to four years of rearing is usually required to reproduce them for the first time. Reproductive control techniques can be applied to repress, delay or advance the onset of puberty. Thus, they can be used to accelerate the process of selective breeding in this species. The present study investigated whether alterations of rearing water temperature (WT) can advance the onset of puberty in the red spotted grouper. Juvenile red spotted grouper (110 DAH, 7.25±0.5 cm, 6.45±1.5 g) were randomly divided into 4 groups and reared for approximately 10 months(from Nov. 2014 to Aug. 2015) at four different WT: natural treatment(12.6-19.5℃), 20℃, 24℃ and 28℃ treatment. When they were reared at 24 or 28℃ WT, sexually mature individuals appeared within 12 months after hatching during their breeding season (Jul. to Aug.). The mRNA levels of reproduction-related genes such as Kisspeptin, GnRH, FSHβ and LHβ were higher at these rearing WT than at natural or 20℃ WT (P<0.05). Mature yolk stage oocytes (≥300 μm diameter) were found in the ovaries of female red spotted grouper reared at 24 or 28℃ WT, while only oogonia were found at natural WT and peri-nucleolus stage oocytes were observed at 20℃ WT, respectively. Moreover, males were found to produce sperm only at 24 or 28℃ WT. The one-year-old mature females ovulated 6-10 ml of eggs that corresponded to 10% of their body weight. In artificial fertilization performed at 24℃ WT, the fertilization and hatching rates were determined to be 95% and 97%, respectively. This is the first report demonstrating that rearing at 24 or 28℃ WT can significantly advance the
onset of puberty in the red spotted grouper.
Author(s)
오승보
Issued Date
2017
Awarded Date
2017. 2
Type
Dissertation
URI
http://dcoll.jejunu.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000008027
Alternative Author(s)
Seung-Bo Oh
Department
대학원 해양생명과학과
Advisor
이영돈
Table Of Contents
목차 Ⅰ
List of figures Ⅲ
List of tables Ⅳ
Abstract . Ⅴ
Ⅰ. 서론 . 1
Ⅱ. 재료 및 방법 3
1. 실험어 및 사육관리 3
2. 실험어 해부 및 조직학적 관찰 . 3
3. Total RNA추출 및 cDNA 합성 4
4. 번식생리관련 유전자의 발현양상 5
5. 인공수정 . 6
6. 통계처리 . 6
Ⅲ. 결과 8
1. 사육 수온 처리구별 생식소 형태와 생식소중량지수(GSI) 8
2. 사육 수온 처리별 생식소발달과 성숙 비율 . 11
3. 사육 수온에 따른 번식생리관련 유전자의 mRNA발현 양상 . 14
4. 성 성숙 개시(Puberty)된 1년생 붉바리의 인공수정 . 16
Ⅳ. 고찰 . 18
Ⅴ. 참고문헌 . 21
Degree
Master
Publisher
제주대학교 일반대학원
Citation
오승보. (2017). 붉바리(Epinephelus akaara)의 성숙 개시와 성 특성
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General Graduate School > Marine Life Sciences
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